• 产品描述： CTB是一种p300激活剂，CTB和p300的最低对接能量为7.72 kcal/mol

• 靶点： p300;EpigeneticReaderDomain

• 体外研究：
  CTB effectively induces apoptosis, up-regulates the expression of P300, and increases the acetylation and total P53 protein levels in MCF-7 cells

• 体内研究：
  CTB combinated with CpG, and aluminum hydroxide playsa synergistic effect to enhance ClfA221-550-induced immune responses, which possesses the potential to develop a novel vaccine to prevent infection of S. aureus

• 细胞实验： Cell lines: MCF-7 and MRC-5 cells Concentrations: 85.43 µM Incubation Time: 24, 48, 72 h Method: MCF-7 and MRC-5 are cultured in RPMI-1640 and treated with or without CTB at a concentration of 85.43 μmol/L at different times (24, 48 and 72 h). The percentage of apoptotic cells are measured by flow cytometry.Real-time quantitative RT-PCR is performed to estimate the mRNA expression of P300 in MCF-7 and MRC-5 with CTB at different times. ELISA and Bradford protein techniques are used to detect levels of total and acetylated P53 protein generated in MCF-7 and MRC-5

• 动物实验： Animal Models: Specific-pathogen-free BALB/c mice Dosages: 100 μg Administration: i.m.

• 参考文献：
  1. Devipriya B, et al. Exploring the binding affinities of p300 enzyme activators CTPB and CTB using docking method. Indian J Biochem Biophys. 2010 Dec;47(6):364-9. 2. Dastjerdi MN, et al. The effect of CTB on P53 protein acetylation and consequence apoptosis on MCF-7 and MRC-5 cell lines. Adv Biomed Res. 2013 Mar 6;2:24. 3. Ma J, et al. The novel combinations of CTB, CpG, and aluminum hydroxide significantly enhanced the immunogenicity of clumping factor A 221-550 of Staphylococcus aureus. Biosci Biotechnol Biochem. 2020 Sep;84(9)

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.909 ml	14.546 ml	29.093 ml
  5 mM	0.582 ml	2.909 ml	5.819 ml
  10 mM	0.291 ml	1.455 ml	2.909 ml
  50 mM	0.058 ml	0.291 ml	0.582 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *