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活死细菌染色试剂盒(可应用流式分析)

    

LIVE/DEAD Bacterial Viability and Counting Kit, for flow cytometry

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R41013 一键复制产品信息
货号 规格 价格 上海 北京 珠海 南京 武汉 购买数量
R41013-100T

¥6300.00

2 - - - -
产品介绍 参考文献(13篇) 质检证书(COA) 摩尔浓度计算器 相关产品

产品介绍

活死细菌染色试剂盒(LIVE/DEAD Bacterial Viability and Counting Kit, for flow cytometry)可以通过流式细胞仪有效的区分活、死细菌并实现定量检测;而且对各类细菌的混合物也可以实现定量分析检测。该试剂盒采用两种核酸染料来进行细菌活力测定:绿色荧光染料(功能等同于SYTO 9)以及红色荧光染料PI(碘化丙啶),并通过标准微球精准测量样品体积。用适当比例的绿色荧光染料与PI混合物染色时,细胞膜完好的细菌发出明亮的绿色荧光,而细胞膜受损的细菌则表现出绿色荧光明显减弱,同时发出较强的红色荧光。细胞类型和革兰氏特征会影响死细菌产生红色荧光的强度。绿色荧光染料与PI染料在大部分流式细胞仪中都是由488nm激光器实现有效激发的,各自的核酸复合物荧光信号可分别在绿色通道和红色通道中检测到,并且几乎没有背景荧光 。 
标准微球悬浮液作为样品体积的参考标准,尺寸和荧光经过仔细筛选,以确保微球在流式细胞检测中荧光--侧向散射图中可以明显的区别于被染色的细菌。

储存条件: 2-8℃
有效期: 12个月
注意: 部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。

参考文献(13篇)

8. [IF=9.5] Zi-Xin Wang et al."Chaotropic Effect-Induced Self-Assembly of the Malachite Green and Boron Cluster for Toxicity Regulation and Photothermal Therapy."ACS Applied Materials & Interfaces.2023;15(48):55486–55494 7. [IF=7.59] Shanshan Lin et al."Hollow silver-gold alloy nanoparticles for enhanced photothermal/photodynamic synergetic therapy against bacterial infection and acceleration of wound healing."Biomaterials Science.2023 May;: 6. [IF=8.025] Zheng Chen et al."Injectable wound dressing based on carboxymethyl chitosan triple-network hydrogel for effective wound antibacterial and hemostasis."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES.2022 Nov;: 5. [IF=6.208] Lanju Xu et al."Preparation of Recombinant Human Collagen III Protein Hydrogels with Sustained Release of Extracellular Vesicles for Skin Wound Healing."INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.2022 Jan;23(11):6289 4. [IF=15.881] Xinyuan Yang et al."Physiologically Inspired Mucin Coated Escherichia coli Nissle 1917 Enhances Biotherapy by Regulating the Pathological Microenvironment to Improve Intestinal Colonization."Acs Nano. 2022;XXXX(XXX):XXX-XXX 3. [IF=6.843] Shilu Ji et al."An oxygen-sensitive probe and a hydrogel for optical imaging and photodynamic antimicrobial chemotherapy of chronic wounds."Biomater Sci-Uk. 2022 Apr;10(8):2054-2061 2. [IF=7.963] Fang Guan et al."Synergistic effect of carbon starvation and exogenous redox mediators on corrosion of X70 pipeline steel induced by Desulfovibrio singaporenus."Sci Total Environ. 2021 Sep;788:147573 1. [IF=8.128] Xiangyong Liu et al."Establishment of anti-oxidation platform based on few-layer molybdenum disulfide nanosheet-coated titanium dioxide nanobelt nanocomposite."J Colloid Interf Sci. 2021 Nov;601:167

质检证书(COA)

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摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

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