产品介绍

活力定义：That amount of enzyme which will catalyze the formation of one micromole of triiodide per minute at 25°C, pH 7.0 in 0.033 M sodium phosphate buffer.

EC 1.11.1.8

简介：The enzyme also catalyzes the oxidation of phenols and aromatics in the presence of hydrogen peroxide. Bovine milk is usually used as a source for isolation and purification of LPO. However, the enzyme is also present in the milk of other species and in the secretions of other mammalian glands such as the salivary gland. LPO from bovine milk has a molecular weight of 77,500. It is a glycoprotein and may exist in two isoenzyme forms (Rombauts, W.A., Schroeder, W.A. and Morrison, M.; Biochemistry, 6, 2965, 1967).

LPO can be used for radioiodination of proteins by coupling the enzyme to cyanogen bromide-activated Sepharose-4B. This coupled form of LPO was capable of iodinating all proteins studied and it exhibited activity over a wide range of experimental conditions (David, G.S., Biochem. Biophys. Res. Comm., 48, 464, 1972).