S80550 |
Zotarolimus |
| MedMol | 98% |
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- 产品描述： Zotarolimus, an analogue of rapamycin, inhibits FKBP-12 (IC50= 2.8 nM).
- 靶点： mTOR
- 体内研究： Zotarolimus potently inhibits adjuvant DTH(ED50=1.72 mg/kg/day), EAE(ED50=1.17 mg/kg/day), and cardiac allograft rejection(ED50=3.71 mg/kg/day)
- 细胞实验： Cell proliferation is assayed by measuring tritiated thymidine incorporation in vitro. Human coronary artery cells (hCa) are seeded into tissue culture flasks for expansion and applied to 96-well plates at desired density in complete media (5000 hCaSMC; 10 000 hCaEC). After 2 days, complete media is replaced with incomplete media to synchronize cells and induce G0 state. Two days later, incomplete media are removed and replaced with complete media (serum/growth factors) to induce G0 to G1 transition. Complete media also contain drug at desired concentrations to determine its effects on cell proliferation. On day 7, 3H-thymidine is added to cells to monitor DNA synthesis, and cells are harvested after overnight incorporation of radioactivity. After an incubation period of 72 h, 25 μL (1 μCi/well) of 3H-thymidine are added to each well. The cells are incubated at 37°C for 16-18 h to allow for incorporation of 3H-thymidine into newly synthesized DNA and the cells harvested onto 96-well plates containing bonded g
- 动物实验： Male Sprague-Dawley rats was administrate by intravenous or oral Zotarolimus（2.5 mg/kg）dissolved inethanol: propylene glycol: cremophor EL: D5W vehicle (20: 30: 2: 48, by volume).
- 参考文献：
1.Chen YW, et al. J Cardiovasc Pharmacol, 2007, 49(4), 228-235. 2.Garcia-Touchard A, et al. Eur Heart J, 2006, 27(8), 988-1993.
- 溶解性： Ethanol:93 mg/mL (96.3 mM) H2O:<1 mg/mL DMSO:93 mg/mL (96.3 mM)
- 保存条件： 2-8℃
- 配置溶液浓度参考：
1mg 5mg 10mg 1 mM 1.035 ml 5.175 ml 10.35 ml 5 mM 0.207 ml 1.035 ml 2.07 ml 10 mM 0.103 ml 0.517 ml 1.035 ml 50 mM 0.021 ml 0.103 ml 0.207 ml
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输入产品批号：
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)
