| 货号 | 规格 | 价格 | 上海 | 北京 | 武汉 | 南京 | 购买数量 |
|---|---|---|---|---|---|---|---|
S80723-2mg |
99.60% | ￥552.00 | 4 | - | - | - |
|
S80723-5mg |
99.60% | ￥850.00 | 6 | - | - | - |
|
S80723-10mg |
99.60% | ￥1480.00 | 5 | - | - | - |
|
S80723-25mg |
99.60% | ￥2460.00 | 6 | - | - | - |
|
S80723-50mg |
99.60% | ￥3600.00 | 5 | - | - | - |
|
S80723-100mg |
≥99% | ￥6120.00 | 货期：2-3天 | - | - | - |
|
| 产品描述： | JNJ-7706621 is a potent aurora kinase inhibitor, and also inhibits CDK1 and CDK2. | ||||||||||||||||||||
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| 靶点： | Apoptosis;Aurora Kinase;CDK;Apoptosis;CDK;AuroraKinase | ||||||||||||||||||||
| 体内研究： | JNJ-7706621在抑制正常细胞类型（包括MRC-5,HASMC,HUVEC和HMVEC）的生长方面具有几倍的效力,IC50为3.67-5.42 μM。JNJ-7706621对CDK1和2具有高度有效性,IC50为3-9 nM。JNJ-7706621显示对包括HeLa,HCT-116,SK-OV-3,PC3,DU145,A375,MDA-MB-231,MES-SA和MES-SA/Dx5在内的一组人类癌细胞类型的抑制作用 ,IC50为112-514 nM,独立于p53,成视网膜细胞瘤或P-糖蛋白状态。JNJ-7706621也抑制CDK3,4和6,IC50为58-253 nM。JNJ-7706621抑制Aurora-A和B,IC50分别为11和15 nM。JNJ-7706621也抑制VEGF-R2,FGF-R2和GSK3β,IC50为154-254 nM。在HeLa或U937细胞中,JNJ-7706621（0.5-3 μM）延迟从G1期中退出,使细胞停滞在G2-M期,诱导核内复制,激活凋亡并减少集落形成 | ||||||||||||||||||||
| 细胞实验： | Cell lines: HeLa,HCT-116,A375,SK-OV-3,MDA-MB-231 and PC-3 cells. Concentrations: 1 nM - 10 μM,dissolved in DMSO. Method: Measuring incorporation of 14C-labelled thymidine into newly synthesized DNA within the cells to determine the ability of JNJ-7706621 to inhibit the proliferation of cell growth.Cells are trypsinized and counted and 3-8 ×103 cells are added to each well of a 96-well CytoStar tissue culture treated scintillating microplate in 100 μL complete medium in a volume.Cells are incubated for 24 hours at 37 °C in an atmosphere containing 5% CO2.Next,1 μL JNJ-7706621 is added to the wells of the plate.Cells are incubated for another 24 hours.Methyl 14C-thymidine 56 mCi/mmol is diluted in complete medium and 0.2 μCi/well is added to each well of the CytoStar plate in a volume of 20 μL.The plate is incubated for 24 hours at 37 °C in JNJ-7706621 with 14C-thymidine.The contents of the plate are discarded and the plate is washed twice with 200 μL PBS.200 μL of PBS is added to each well.The top of the plate | ||||||||||||||||||||
| 动物实验： | Animal Models: Mouse xenograft model of A375 cellsFormulation & . Dosages: Dissolved in 0.5% methylcellulose containing 0.1% polysorbate 80 in sterile water.100 or 125 mg/kg. Administration: Orally or by intraperitoneal injection | ||||||||||||||||||||
| 参考文献： | 1.Seamon JA, et al. Mol Cancer Ther, 2006, 5(10), 2459-2467. 2.Lin R, et al. J Med Chem, 2005, 48(13), 4208-4211. 3.Emanuel S, et al. Cancer Res, 2005, 65(19), 9038-9046. | ||||||||||||||||||||
| 溶解性： | Ethanol:<1 mg/mL DMSO:73 mg/mL (185.1 mM) H2O:<1 mg/mL | ||||||||||||||||||||
| 保存条件： | -20℃ | ||||||||||||||||||||
| 配置溶液浓度参考： |
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| 注意： | 部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
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