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• 产品描述： Methylene Blue (Basic Blue 9, Tetramethylthionine chloride, methylthioninium chloride, CI-52015)在色素内镜检查中被作用染料。它能抑制tau蛋白丝的形成，IC50为1.9μM，同时还抑制可溶性鸟苷环化酶

• 靶点： guanylate cyclase; Tau aggregation:1.9 μM

• 体外研究： Methylene Blue可能抑制NO合成酶或通过产生超氧阴离子，直接使一氧化氮失活。除了干扰一氧化氮换鸟苷酸途径，Methylene Blue直接激活钙离子依赖性的钾通道来增强去甲肾上腺素从细胞内的释放

• 体内研究： Methylene Blue抑制鸟苷酸环化酶，减少C-GMP和血管平滑松弛。当进行静脉注射时，亚甲蓝在甲状旁腺积累，从而在外科手术中借以其颜色来判断这些内部结构。在啮齿类的药动学实验揭示，血管内进行MB给药将导致MB在中枢神经细胞中迅速广泛的积累

• 细胞实验： Cell lines: 未成熟的成神经细胞 Concentrations: 10 μM Incubation Time: 2 h Method: 从新生大鼠的室管膜下层分离出来的未成熟的成神经细胞，在单细胞培养模型中，分化成γ氨基丁酸所介导神经元，用此神经元细胞用于后续实验。在体外实验中，第6天，用MB对细胞培养物进行处理2h，处理完48小时后，检测细胞树突分枝结构及生存情况

• 动物实验： Animal Models: Sprague- Dawley雄性大鼠 Dosages: 5 and 50 mg/kg Administration: 血管内注射

• 参考文献：
  1. Vutskits L, et al. Adverse effects of methylene blue on the central nervous system. Anesthesiology. 108(4):684-692. 2. Hochgr鋐e K, et al. Preventive methylene blue treatment preserves cognition in mice expressing full-length pro-aggregant human Tau. Acta Neuropathol Commun. 2015, 3:25. 3. Masaki E, et al. Methylene blue, a soluble guanylyl cyclase inhibitor, reduces the sevoflurane minimum alveolar anesthetic concentration and decreases the brain cyclic guanosine monophosphate content in rats. Anesth Analg. 1999, 89(2):484-489. 4. Ginimuge PR, et al. Methylene blue: revisited. J Anaesthesiol Clin Pharmacol. 2010, 26(4):517-520.

• 溶解性： Soluble  in  DMSO、Ethanol、H2O

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	3.126 ml	15.632 ml	31.265 ml
  5 mM	0.625 ml	3.126 ml	6.253 ml
  10 mM	0.313 ml	1.563 ml	3.126 ml
  50 mM	0.063 ml	0.313 ml	0.625 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *