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• 产品描述： EMD638683 是一种 serum- and glucocorticoid-inducible kinase 1 (SGK1) 的高度选择性抑制剂，其IC50值为3 μM。EMD638683 具有降压药效和抗肿瘤活性

• 靶点： SGK1(Cell-free assay):3 μM

• 体内研究： The in vivo development of tumors following chemical carcinogenesis is significantly blunted by treatment with EMD638683. Within 24 hours in vivo EMD638683 treatment significantly decreases blood pressure in fructose/saline-treated mice but not in control animals or in SGK1 knockout mice

• 细胞实验： Cell lines: HeLa-cells Concentrations: 0.1 μM, 0.3 μM, 1 μM, 3 μM, 10 μM Incubation Time: 24 h Method: The cells are plated in 6-wells MTPs at a density of 10–20 × 103 cells/cm2 in Dulbecco´s DMEM medium, supplemented with 10% fetal calf serum (FCS), 2 mM glutamine and 1 mM sodium pyruvate. After 24 hrs at 37℃ and 5% CO2 in a cell incubator, each well is further supplemented with 25 μl of a 100X dimethyl sulfoxide (DMSO) solution of the compound; the solution is diluted 100-fold in the supernatant of the cell culture, thus resulting in the anticipated SGK1-inhibitor concentration at a 1% DMSO concentration.

• 动物实验： Animal Models: 8-week-old wild type mice Dosages: 20 mg/kg, 600 mg/kg Administration: IP, Oral gavage

• 参考文献：
  1. Teresa F Ackermann, et al. Cell Physiol Biochem. 2011;28(1):137-46. 2. Syeda T Towhid, et al. Cell Physiol Biochem. 2013;32(4):838-48.

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.745 ml	13.723 ml	27.447 ml
  5 mM	0.549 ml	2.745 ml	5.489 ml
  10 mM	0.274 ml	1.372 ml	2.745 ml
  50 mM	0.055 ml	0.274 ml	0.549 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *