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- 产品描述： JNK-IN-7 is a selective JNK1/2/3 inhibitor (IC50: 1.54/1.99/0.75 nM). It can also inhibit phosphorylation of c-Jun, which is a substrate of JNK kinase.
- 靶点： JNK
- 细胞实验： JNK-IN-7 is prepared in DMSO and stored, and then diluted with appropriate medium before use. Intestinal epithelial cell line (HCT116) is cultured in DMEM medium, supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin (100U/mL) and streptomycin (100g/mL), 2mM L-gentamycin, and 50μM 2-ME. These cells are stimulated with TNF (20ng/mL), LPS (100ng/mL), and IFN-γ (20ng/mL), respectively. After 24 or 48h of culture, cells are harvested followed by extraction of total RNA, and the levels of DMT1 mRNA are analyzed by qRT-PCR. To determine the mechanisms of TNF involved in regulating DMT1 expression, JNK-IN-7 (1μM), NF-κB inhibitor (BAY 11-7082, 1μM), and caspase-3/8 inhibitor (Z-DEVD-FMK, 50μM) are also added into the culture medium. After 48h of culture, cells are then collected to detect the expression of DMT1 by qRT-PCR
- 参考文献：
1. Wu W, et al. Divalent metal-ion transporter 1 is decreased in intestinal epithelial cells and contributes to the anemia in inflammatory bowel disease. Sci Rep. 2015 Nov 17;5:16344. 2. Xu P, Zeng S, Xia X, et al. FAM172A promotes follicular thyroid carcinogenesis and may be a marker of FTC[J]. Endocrine-Related Cancer. 2020, 1(aop). 3. Zhang T, et al. Discovery of Potent and Selective Covalent Inhibitors of JNK. Chem Biol. 2012 Jan 27;19(1):140-54.
- 溶解性： Soluble in DMSO
- 保存条件： -20℃
- 配置溶液浓度参考：
1mg 5mg 10mg 1 mM 2.026 ml 10.13 ml 20.261 ml 5 mM 0.405 ml 2.026 ml 4.052 ml 10 mM 0.203 ml 1.013 ml 2.026 ml 50 mM 0.041 ml 0.203 ml 0.405 ml
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)
