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• 产品描述： Yoda 1 is a potent and selective Piezo1 agonist. Yoda 1 activates purified Piezo1 channels. Yoda 1 potently inhibits macropinocytosis induced by epidermal growth factor (EGF). Yoda 1 enhances Ca2+ influx followed by activation of the calcium-activated potassium channel KCa3.1 and inhibition of Rac1 activation

• 靶点： ERK1;ERK2

• 体外研究： Yoda1 (0-6 μM, 5 min) induces the activation of both Akt and ERK1/2, which is not dependent on Piezo1. Yoda1 (1.5 μM, 5 min) inhibits Rac1 activation. Western Blot Analysis Cell Line: human coronary artery endothelial cells (HCAECs)[2], A431 cells Concentration: 0, 1.5, 3.0, and 6.0 μM Incubation Time: 5 min Result: Induced the activation of both Akt and ERK1/2, and increased the phosphorylation levels of both Akt and ERK1/2 in a dose-dependent manner. Inhibited EGF-induced increase in the amount of Rac1-GTP, and inhibited Rac1 activation

• 参考文献：
  1. Syeda R, et al. Chemical activation of the mechanotransduction channel Piezo1. Elife. 2015 May 22;4. 2. Dela Paz NG, et al. Yoda1-induced phosphorylation of Akt and ERK1/2 does not require Piezo1 activation. Biochem Biophys Res Commun. 2018 Feb 26;497(1):220-225. 3. Kuriyama M, et al. Piezo1 activation using Yoda1 inhibits macropinocytosis in A431 human epidermoid carcinoma cells. Sci Rep. 2022 Apr 15;12(1):6322.

• 溶解性： Soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.815 ml	14.074 ml	28.148 ml
  5 mM	0.563 ml	2.815 ml	5.63 ml
  10 mM	0.281 ml	1.407 ml	2.815 ml
  50 mM	0.056 ml	0.281 ml	0.563 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *