货号	规格	价格	上海	北京	珠海	武汉
S87651-5mg	99.40%	￥980.00 S87651-5mg ￥980.00 现货	1 1	-	-	-
S87651-10mg	99.40%	￥1500.00 S87651-10mg ￥1500.00 现货	4 4	-	-	-
S87651-25mg	99.40%	￥2900.00 S87651-25mg ￥2900.00 现货	4 2 4	2	-	-
S87651-100mg	99.40%	￥5800.00 S87651-100mg ￥5800.00 现货	6 6	-	-	-

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产品描述：

D-Lin-MC3-DMA, an ionizable cationic lipid, is a potent siRNA delivery vehicle.

体外研究：

Replacement of DSPC by unsaturated PCs and DLin-MC3-DMA by the related lipid DLin-KC2-DMA resulted in highly potent transfection reagents for HeLa cells in vitro

体内研究：

含有二硬脂酰磷脂酰胆碱 (DSPC) 和可电离氨基脂质（如二亚油基甲基-4-二甲氨基丁酸酯 (DLin-MC3-DMA)）的脂质纳米颗粒 (LNP) 是有效的体内 siRNA 递送载体。LNP-siRNA 系统经过优化，可在小鼠静脉注射后在肝细胞中实现最大的基因沉默效力，其中包含 DLin-MC3-DMA (MC3)、DSPC、胆固醇和聚乙二醇 (PEG)-脂质，摩尔比为 50/10/38.5/1.5。DLin-MC3-DMA 表现出良好的的 pKa 值，可显著提高效力。

细胞实验：

White leghorn chicken embryos at stage 24 (30) were removed and dissected in Hanks buffered saline solution (HBSS).?Forelimbs were pooled and treated for 1 h in dispase to remove epithelial ectoderm.?Limb mesenchyme was dissociated, and resuspended in DMEM with 5% FBS and 1% penicillin/streptomycin.?Cells were plated onto 96-well plates at a density of 2x10^5 or 2x10^7 cells/ml and treated with 0-40 μg/ml LNP-pDNA.?Cultures were incubated overnight at 37℃ and resuspended in PBS buffer for expression analysis using a BD LSRII flow cytometer.?Cell survival was assessed in low-density cultures after 24 h of treatment, by counting the adherent cells remaining after PBS washes.?Viability was determined based on average counts of all adherent cells within a single field of view (100x) normalized to cell counts of untreated cultures.

参考文献：

1. Kulkarni J A , Myhre J L , Chen S , et al. Design of lipid nanoparticles for, in vitro, and, in vivo, delivery of plasmid DNA[J]. Nanomedicine: Nanotechnology, Biology and Medicine, 2017, 13(4):1377-1387.

溶解性：

Soluble in DMSO、Ethanol

保存条件：

-20℃

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	1.557 ml	7.787 ml	15.574 ml
5 mM	0.311 ml	1.557 ml	3.115 ml
10 mM	0.156 ml	0.779 ml	1.557 ml
50 mM	0.031 ml	0.156 ml	0.311 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

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