• 产品描述： DLin-KC2-DMA is an ionisable cationic lipid (pKa≈6) that is virtually non-toxic to antigen presenting cells (APCs). DLin-KC2-DMA produces significant siRNA-mediated gene silencing of GAPDH, when binds to lipid nanoparticles (LNP). DLin-KC2-DMA can be used in siRNA delivery studies

• 靶点： Liposome

• 体外研究：
  DLin-KC2-DMA (1, 5 μg; 72 h) effectively produces a significant siRNA-mediated GAPDH gene silencing in both macrophages and dendritic cells. DLin-KC2-DMA (24 h) exhibits high uptake into macrophages and dendritic cells. DLin-KC2-DMA efficiently promotes release of encapsulated siRNA into the cytosol following uptake via the endocytotic pathway. DLin-KC2-DMA displays almost no toxicity in primary APCs. Cell Cytotoxicity Assay Cell Line: Macrophages, dendritic cells Concentration: 5 μg/mL (DLin-KC2-DMA contained-LNPs) Incubation Time: 72 h Result: Displayed almost no toxicity. Western Blot Analysis Cell Line: Macrophages, dendritic cells Concentration: 1, 5 μg Incubation Time: 72 h Result: Exhibited significant GAPDH silencing of more than 60% at 1 μg and of 80% at 5 μg in macrophages.Significantly reduced GAPDH protein and exhibited the silencing effects of 83% at 5 μg.

• 体内研究：
  DLin-KC2-DMA contained-LNP siRNA systems (5 mg/kg; i.v.; single) effectively silences target genes in APcs in vivo. Animal Model: Naive C57BL/6 mice. Dosage: 3 or 5 mg/kg (DLin-KC2-DMA contained-LNPs) Administration: Intravenous injection; single. Result: Significantly reduced GAPDH production in peritoneal cavity macrophages and dendritic cells and in the spleen-derived APCs when at 5 mg/kg.

• 参考文献：
  1. Basha G, et al. Influence of cationic lipid composition on gene silencing properties of lipid nanoparticle formulations of siRNA in antigen-presenting cells. Mol Ther. 2011 Dec;19(12):2186-200. 2. Miller AD, et al. Delivery of RNAi therapeutics: work in progress. Expert Rev Med Devices. 2013 Nov;10(6):781-811.

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存条件： -20℃，充氮保存

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	1.557 ml	7.787 ml	15.574 ml
  5 mM	0.311 ml	1.557 ml	3.115 ml
  10 mM	0.156 ml	0.779 ml	1.557 ml
  50 mM	0.031 ml	0.156 ml	0.311 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

输入产品批号：

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *