• 产品描述： Dyngo-4a是一种有效的dynamin抑制剂，作用于DynI (brain)，DynI (rec)，和DynII (rec)，IC50分别为0.38 μM，1.1 μM，和 2.3 μM

• 靶点： DynI (brain):0.38 μM;DynI (rec):1.1 μM;DynII (rec):2.3 μM

• 细胞实验： Cell lines: αT3–1 or LβT2 cells Concentrations: 30 μM Incubation Time: 30 minutes Method: αT3-1 or LβT2 cells (2 × 106) were grown overnight in a 6-well culture dish and then serum starved for 2–4 hours. Cells were pretreated with either vehicle (0), dynasore (80μM), or dyngo (30μM) for 30 minutes. For dose-response studies, indicated doses of dynasore were used for a 30-minute pretreatment. After pretreatment, cells were treated with 0 or GnRHa (10 nM) for 10 minutes. Cells were then washed twice in PBS, lysed in radio-immunoprecipitation assay (RIPA) buffer and subjected to SDS-PAGE (acrylamide:bis-acrylamide ratio of 29:1) and electroblotted to polyvinylidene difluoride membranes.

• 动物实验： Animal Models: Female CD-1 mice Dosages: 30 mg/kg Administration: i.p.

• 参考文献：
  1. McCluskey A, et al. Traffic. 2013, 14(12), 1272-1289. 2. Harper CB, et al. J Biol Chem. 2011, 286(41), 35966-35976. 3. Gagliardi M, et al. J Cell Sci. 2014, 127(22), 4918-4926. 4. Harper CB, et al. J Biol Chem. 2011, 286(41):35966-76. 5. Edwards BS, et al. Endocrinology. 2016, 157(2):831-43.

• 溶解性： Soluble  in  DMSO、Ethanol

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	2.956 ml	14.779 ml	29.559 ml
  5 mM	0.591 ml	2.956 ml	5.912 ml
  10 mM	0.296 ml	1.478 ml	2.956 ml
  50 mM	0.059 ml	0.296 ml	0.591 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *