TPEN

    
98%

TPEN

源叶(MedMol)
S88052 一键复制产品信息
16858-02-9
C26H28N6
424.54
N,N,N',N'-四(2-吡啶甲基)乙二胺
货号 规格 价格 上海 北京 武汉 南京 购买数量
S88052-50mg 98% ¥425.00 >10 - - -
S88052-100mg 98% ¥637.50 >10 - - -
S88052-250mg 98% ¥1020.00 >10 - - -
产品介绍 参考文献(1篇) 质检证书(COA) 摩尔浓度计算器 相关产品

产品介绍

TPEN is a specific cell-permeable heavy metal chelator.

产品描述: TPEN is a specific cell-permeable heavy metal chelator.
靶点: Apoptosis;Autophagy;Reactive Oxygen Species;Apoptosis; ReactiveOxygenSpecies; Autophagy
细胞实验: TPEN is dissolved in DMSO and then diluted with appropriate medium[1]. Human neuroblastoma cell line SH-SY5Y, are grown in Dulbecco's Modified Eagle's Medium (DMEM) mixed 1:1 with Ham's F-12 nutrient mixture containing 10% fetal bovine serum, 100 unit/mL penicillin and 100 μg/mL streptomycin at 37°C in a humidified 5% CO2 atmosphere. Two days before experimentation, cells are seeded at a density of 7×104 cells/cm2 in a 96-well plate. Cells in a 96-well plate are serum-starved for 4 hr; calcium indicator fura-2 is then loaded into the cells by using Calcium kit II fura-2. In brief, SH-SY5Ycells are incubated with 5 μM fura-2/AM in the presence of 0.04% Pluronic F-127, a dispersing agent to improve the efficiency of loading with fura-2, and 1.25 mM probenecid, a blocker of organic anion transport to prevent leakage of fura-2 from cells. After 1 hr incubation at 37°C, fura-2 fluorescence is measured at 500 nm emission after excitation at 340 nm (F340) or 380 nm (F380) using an Infinite M200 plate reader at 37°C.
参考文献: 1. Rahal ON, et al. Chk1 and DNA-PK mediate TPEN-induced DNA damage in a ROS dependent manner in human colon cancer cells. Cancer Biol Ther. 2016 Nov;17(11):1139-1148. 2. Ohkubo M, et al. Heavy metal chelator TPEN attenuates fura-2 fluorescence changes induced by cadmium, mercury and methylmercury. J Vet Med Sci. 2016 Jun 1;78(5):761-7.
溶解性: Soluble  in  DMSO
保存条件: -20℃
配置溶液浓度参考:
1mg 5mg 10mg
1 mM 2.355 ml 11.777 ml 23.555 ml
5 mM 0.471 ml 2.355 ml 4.711 ml
10 mM 0.236 ml 1.178 ml 2.355 ml
50 mM 0.047 ml 0.236 ml 0.471 ml
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