S89194 |
MCU-i4 |
| 源叶(MedMol) | 98% |
- 产品描述： MCU-i4 是 mitochondrial calcium uniporter (MCU) 复合物的负调节剂，可直接结合 MICU1 中的特定裂缝并减少线粒体 Ca2+ 流入
- 靶点： MCU1
- 体外研究： MCU-i4 decreases mitochondrial Ca2+ influx. Docking simulations reveal that MCU-i4 directly binds a specific cleft in MICU1, a key element of the MCU complex that controls channel gating. Accordingly, in MICU1-silenced or deleted cells, the inhibitory effect of MCU-i4 is lost. Moreover, MCU-i4 fails to inhibit mitochondrial Ca2+ uptake in cells expressing a MICU1 mutated in the critical amino acids that forge the predicted binding cleft.
- 细胞实验： Cell lines: C2C12 myoblasts Concentrations: 10 μM Incubation Time: 24 h Method: Growing medium of 90% confluent C2C12 myoblasts is replaced with DMEM containing 2%-heat inactivated horse serum (HS) (differentiation medium). Five days later, cells are incubated for 24 h with MCU-i4 (10 μM) or with DMSO alone (0.1% v/v). At the end of the experiment, myotubes images are taken and myotubes width is measured by means of ImageJ software.
- 参考文献：
1. Giulia Di Marco, et al. A High-Throughput Screening Identifies MICU1 Targeting Compounds. Cell Rep. 2020 Feb 18;30(7):2321-2331.e6.
- 溶解性： Soluble in DMSO、Ethanol
- 保存条件： -20℃
- 配置溶液浓度参考：
1mg 5mg 10mg 1 mM 2.651 ml 13.255 ml 26.51 ml 5 mM 0.53 ml 2.651 ml 5.302 ml 10 mM 0.265 ml 1.326 ml 2.651 ml 50 mM 0.053 ml 0.265 ml 0.53 ml
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质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)
