• 产品描述： SPP-86 is a potent and selective cell permeable inhibitor of RET tyrosine kinase, with an IC50 of 8 nM. SPP-86 inhibits RET-induced phosphatidylinositide 3-kinases (PI3K)/Akt and MAPK signaling, also inhibits RET-induced estrogen receptorα (ERα) phosphorylation in MCF7 cells

• 靶点： IC50: 8 nM (RET)

• 体外研究： SPP86 (0-10 μM) inhibits MAPK signaling and proliferation in RET/PTC1 expressing TPC1 but not 8505C or C643 cells. SPP86 (0-10 μM) inhibits RET- induced phosphatidylinositide 3-kinases (PI3K)/Akt and MAPK signaling and estrogen receptorα (ERα) phosphorylation in MCF7 cells. Western Blot Analysis Cell Line: Thyroid cancer derived cell lines expressing the RET/PTC1 rearrangement (TPC1), BRAFV600E (8505C) or RASG13R (C643) mutations. Concentration: 0-10 μM. Incubation Time: 90 min. Result: Inhibited RET- induced ERK1/2 phosphorylation in thyroid cancer cell lines. Western Blot Analysis Cell Line: MCF7 cells (human breast cancer). Concentration: 0-10 μM. Incubation Time: 30 min. Result: Inhibited RET- induced ERα phosphorylation and proliferation in MCF7 cells.

• 参考文献：
  1. Alao JP, Michlikova S, Dinér P, Grøtli M, Sunnerhagen P. Selective inhibition of RET mediated cell proliferation in vitro by the kinase inhibitor SPP86. BMC Cancer. 2014 Nov 20;14:853.

• 溶解性： Soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	3.606 ml	18.029 ml	36.058 ml
  5 mM	0.721 ml	3.606 ml	7.212 ml
  10 mM	0.361 ml	1.803 ml	3.606 ml
  50 mM	0.072 ml	0.361 ml	0.721 ml

• 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *