• 产品描述： Pyroxamide is an histone deacetylases (HDACs) inhibitor with antineoplastic properties ( HDAC1;IC50: 0.1-0.2 μM).

• 靶点： Apoptosis;HDAC

• 体内研究： Pyroxamide may be a useful agent for the treatment of malignancy and that induction of p21/WAF1 in transformed cells by pyroxamide may contribute to the antitumor effects of this agent

• 细胞实验： HDAC1 enzyme assay, A MEL cell line expressing the epitope Flag-tagged HDAC1 was generated.HDAC1-Flag was affinity purified by immunoprecipitation using M2 anti-Flag antibody-coated agarose, followed by elution from the agarose using the Flag peptide.[3H]acetate-labeled cellular histones were prepared from MEL cells and were used as a substrate for the HDAC activity assay.Released [3H]acetic acid was quantified by scintillation counting.For inhibition studies, the enzyme preparations were preincubated with pyroxamide (10 to 100,000 nm) for 30 min at 4°C.

• 参考文献：
  1. Butler L M , Webb Y , Agus D B , et al. Inhibition of transformed cell growth and induction of cellular differentiation by pyroxamide, an inhibitor of histone deacetylase[J]. Clinical Cancer Research, 2001, 7(4):962-970.

• 溶解性： Soluble  in  DMSO

• 保存条件： -20℃

• 配置溶液浓度参考：
  

  	1mg	5mg	10mg
  1 mM	3.769 ml	18.846 ml	37.692 ml
  5 mM	0.754 ml	3.769 ml	7.538 ml
  10 mM	0.377 ml	1.885 ml	3.769 ml
  50 mM	0.075 ml	0.377 ml	0.754 ml

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *