产品描述: | Cy5-SE (Cy5 NHS Ester) triethylamine salt 一种用于标记肽, 蛋白质, 寡核苷酸的氨基基团的反应染料 (Ex=649 nm; Em=670 nm)。 |
体外研究: |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Conjugation of SELP Analogues with Cy5-SE. 1. 1 mL of each 100 μg/mL SELP solution in 100 mM sodium bicarbonate buffer (pH 8.3) is mixed with 10 μL of 1.2 mg/mL Cy5 mono NHS-ester in 10% DMSO and incubates for 2 hours on ice. 2. To quench the reaction, 50 μL of 1 M Tris-HCl (pH 8.0) is added to the reaction solution. 3. Reaction mixtures are loaded onto 1.5 mL Sephadex G-25 columns, and Cy5-conjugated SELPs are eluted by centrifugation for 3 minutes at 1050g. |
参考文献: |
1. Jeon HY, et al. Array-Based High-Throughput Analysis of Silk-Elastinlike Protein Polymer Degradation and C-Peptide Release by Proteases. Anal Chem. 2016;88(10):5398-5405. |
保存条件: |
-20℃,干燥避光 |
配置溶液浓度参考: |
|
1mg |
5mg |
10mg |
1 mM |
1.169 ml |
5.847 ml |
11.695 ml |
5 mM |
0.234 ml |
1.169 ml |
2.339 ml |
10 mM |
0.117 ml |
0.585 ml |
1.169 ml |
50 mM |
0.023 ml |
0.117 ml |
0.234 ml |
|
注意: |
部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。 |