| 产品名称: |
Recombinant EN-TEV Protease Protein |
| 规格或纯度: |
无载体, 生物活性, ActiBioPure™, 无叠氮钠, ≥98%(SDS-PAGE), 见COA |
| 产品介绍: |
Carrier free, >98% (SDS-PAGE), Active, E.coli, N-His tag, 2038-2279 aa |
| 应用: |
SDS-PAGE;Functional Studies |
| 生物活性: |
Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser , with the cleavage point after Gln. One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.Complete digestion of 50µg of fusion KGF-MBP tag protein using 1µg TEV enzyme (in 1/50 ratio) in either 1 hours at 30 °C or overnight at 4 °C. The 'standard' reaction buffer for TEV protease is 50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT. |
| 内毒素浓度: |
<1.0 EU/μg |
| 表达系统: |
E. coli |
| 种属: |
Tobacco etch virus(TEV) |
| 氨基酸: |
2038-2279 aa |
| 序列: |
GHHHHHHHGESLFKGPRDYNPISSSICHLTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLVVQSLHGVFKVKDTTTLQQHLVDERDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSSTFPSGDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFANTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQLMNRRRRR |
| 蛋白标签: |
N-His |
| 蛋白长度: |
Protein fragment |
| 无载体: |
Yes |
| Accession ID: |
P04517 |
| 来源: |
Recombinant |
| 预测分子量: |
28.6 kDa |
| 物理外观: |
液体 |
| 储存缓冲液: |
25mM HEPES buffer, 400mM NaCl, PH7.5 |
| 浓度: |
见COA |
| 储存温度: |
-20°C储存;避免反复冻融 |
| 运输条件: |
超低温冰袋运输 |
| 稳定性和储存: |
在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。 |
| 注意: |
部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。 |