产品介绍
铁是人体必须的微量元素之一,也是血红蛋白、肌红蛋白、细胞色素及其他酶系统的主要成分,在氧的运输和脂肪氧化过程中起着重要作用,铁元素缺乏易造成贫血、代谢紊乱并影响机体免疫功能,铁过量是引发或加剧多种慢性病(如糖尿病、心脑血管疾病、神经退化性疾病等)的危险因素。
该试剂盒的检测原理是亚铁离子(Fe²⁺)在酸性条件下能够与三吡啶基三嗪(TPTZ)形成蓝色配合物,产物在593nm处具有特征吸收峰,通过酶标仪测定吸光值的变化即可定量检测植物组织、动物、血清、细胞等样本中Fe²⁺的含量。本产品仅用于科研领域,不适用于临床诊断或其他用途。
Iron is an essential trace element in the human body and a key component of hemoglobin, myoglobin, cytochrome, and various enzyme systems. It plays a crucial role in oxygen transport and fatty acid oxidation. Iron deficiency can lead to anemia, metabolic disorders, and impaired immune function, while iron overload is a risk factor for the development or progression of several chronic diseases, such as diabetes, cardiovascular disorders, and neurodegenerative conditions.
The detection principle of this kit is based on the formation of a blue complex between ferrous ion (Fe²⁺) and tripyridyltriazine (TPTZ) under acidic conditions. The resulting product exhibits a characteristic absorption peak at 593 nm. By measuring the change in absorbance with a microplate reader, the content of Fe²⁺ in samples such as plant tissues, animal specimens, serum, and cells can be quantitatively determined. This product is intended for research use only and is not suitable for clinical diagnosis or other purposes.