产品介绍
是否无菌:是本产品是专门为Protein A/G亲和色谱介质纯化抗体(IgG)而设计的结合缓冲液。其适宜的pH值和离子强度能确保多种物种来源(如人、兔、小鼠、大鼠等)的IgG抗体与Protein A/G介质发生特异性、高亲和力的结合,同时最大限度地减少非特异性杂蛋白的结合,为获得高纯度、高得率的抗体提供最佳环境。使用时需用去离子水将10x缓冲液稀释至1x,具体使用方法可根据不同纯化工具进行使用。
This product is a binding buffer specifically designed for the purification of antibodies (IgG) using Protein A/G affinity chromatography media. Its optimal pH and ionic strength ensure specific, high-affinity binding of IgG antibodies from various species (such as human, rabbit, mouse, rat, etc.) to the Protein A/G media, while minimizing non-specific binding of impurities. This provides an ideal environment for obtaining antibodies with high purity and yield.Prepare 1× Binding Buffer by diluting Binding Buffer(10×) with deionized water.The specific method of use may vary depending on the purification tool employed.