Protein A/G 琼脂糖填料蛋白洗脱缓冲液 (5×)，pH 2.8

产品介绍

是否无菌：是 本产品是一种专为Protein A、Protein G、Protein A/G抗体纯化柱料的洗脱而设计的缓冲液。其低pH环境（pH 2.5-3.0）能够有效破坏Protein A/G与IgG抗体Fc段之间的高亲和力相互作用，从而将抗体从柱料上解离下来，实现高效洗脱。本缓冲液适用于纯化多种物种来源（如人、兔、小鼠、大鼠等）的多克隆或单克隆抗体。使用时需用去离子水将5x缓冲液稀释至1x，具体
使用方法可根据不同纯化工具进行使用。
This product is a buffer specifically designed for the elution of antibodies from Protein A, Protein G, and Protein A/G purification columns. Its low-pH environment (pH 2.5-3.0) effectively disrupts the high-affinity interaction between Protein A/G and the Fc region of IgG antibodies, thereby dissociating the antibodies from the column matrix and achieving efficient elution. This buffer is suitable for purifying polyclonal or monoclonal antibodies derived from various species, such as human, rabbit, mouse, rat, and others.Prepare 1X Protein A/G Elution Buffer by diluting Protein Protein A/G Buffer(5X) with deionized water. The specific method of use may vary depending on the purification tool employed.