吖啶橙染色试剂盒

产品介绍

吖啶橙(Acridine Orange, AO)是一种异染性荧光染料，其荧光颜色因结合物不同而变化：与双链DNA结合：嵌入碱基对间，激发后发射绿色荧光（Ex 488nm, Em 530nm）。与单链RNA或溶酶体结合：静电吸附，发射橙红色荧光（Em＞640nm）。在荧光显微镜下观察，吖啶橙可透过正常细胞膜，使细胞核呈绿色或黄绿色均匀荧光；而在凋亡细胞中，因染色质固缩或断裂为大小不等的片断，形成凋亡小体。吖啶橙使其染上致密浓染的黄绿色荧光，或黄绿色碎片颗粒；而坏死细胞黄绿荧光减弱甚至消失。吖啶橙(AO)常与
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