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菠萝蛋白酶

    
BR,300u/mg

Bromelaine

源叶
S10009 一键复制产品信息
37189-34-7
MFCD00081423
菠萝酶;风梨酶
货号 规格 价格 上海 北京 珠海 重庆 武汉 购买数量
S10009-25g
BR,300u/mg

¥70.00

>10 - 7 - 9
S10009-100g
BR,300u/mg

¥220.00

8 2 2 - -
S10009-500g
BR,300u/mg

¥560.00

9 - 3 - -
产品介绍 参考文献(70篇) 质检证书(COA) 摩尔浓度计算器 相关产品

产品介绍

菠萝蛋白酶是从凤梨属植物-菠萝中经生物工程技术提取精制而成的一组巯基水解蛋白酶。菠萝蛋白酶主要来源于植物茎中,故也称为茎菠萝蛋白酶。菠萝蛋白酶的主要成分是一种含巯基的蛋白酶,同时还包含过氧化物酶、酸性磷酸酯酶、几种蛋白抑制剂和有机活性钙,其活性中心为巯基(—SH),能进行各种蛋白质水解,进行生化反应,可广泛应用于食品、医药及生物等行业。

分子量:33000 。

等电点:9.55 。

最适合作用温度:50~60℃。

失活温度:65℃。

适宜PH:5.0-8.0。

失活PH:低于 3.0 和高于 9.5。

性状:浅黄色粉末,有轻微的菠萝特殊气味。

酶活定义:紫外光分光度法:在测定条件(37±0.2℃;PH值7.0)每分钟水解酪蛋白释出的三氯乙酸可溶物在275nm波长有吸光度与1微克酪氨酸的吸光度相当时,所需的酶量即为一个活力单位,用u/g表示。

 

溶解性: 产品本身带有不溶性载体,直接使用L-半胱氨酸盐酸盐缓冲液配制,研磨搅拌或涡旋20-30min,离心取上清液或使用即可,巯基为产品激活剂。缓冲液配制步骤:称取  L-半胱氨酸盐酸盐5.27  g,氯化钠23.4g,加水  500  mL  溶解,另取乙二胺四乙酸二钠2.23g,加水  200  mL,溶解,合并两液混匀,用  0.1mol/L  氢氧化钠溶液或  0.1  mol/L盐酸溶液调至  pH=5.5,加水定容至  1000  mL。
储存条件: 2-8℃
EC编号: 3.4.22.32
注意: 部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。

参考文献(70篇)

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摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

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