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菠萝蛋白酶

    
生物技术级,600u/mg

Bromelaine

源叶
S10010 一键复制产品信息
37189-34-7
MFCD00081423
菠萝酶;风梨酶
货号 规格 价格 上海 北京 珠海 重庆 武汉 购买数量
S10010-25g
生物技术级,600u/mg

¥280.00

9 - 1 - -
S10010-100g
生物技术级,600u/mg

¥480.00

2 - - - -
S10010-500g
生物技术级,600u/mg

¥1000.00

10 6 - - -
产品介绍 参考文献(55篇) 质检证书(COA) 摩尔浓度计算器 相关产品

产品介绍

 菠萝蛋白酶是从凤梨属植物-菠萝中经生物工程技术提取精制而成的一组巯基水解蛋白酶。菠萝蛋白酶主要来源于植物茎中,故也称为茎菠萝蛋白酶。菠萝蛋白酶的主要成分是一种含巯基的蛋白酶,同时还包含过氧化物酶、酸性磷酸酯酶、几种蛋白抑制剂和有机活性钙,其活性中心为巯基(—SH),能进行各种蛋白质水解,进行生化反应,可广泛应用于食品、医药及生物等行业。

分子量:33000 。

等电点:9.55 。

最适合作用温度:50~60℃。

失活温度:65℃。

适宜PH:5.0-8.0。

失活PH:低于 3.0 和高于 9.5。

性状:浅黄色粉末,有轻微的菠萝特殊气味。

酶活定义:紫外光分光度法:在测定条件(37±0.2℃;PH值7.0)每分钟水解酪蛋白释出的三氯乙酸可溶物在275nm波长有吸光度与1微克酪氨酸的吸光度相当时,所需的酶量即为一个活力单位,用u/g表示。

溶解性: 产品本身带有不溶性载体,直接使用L-半胱氨酸盐酸盐缓冲液配制,研磨搅拌或涡旋20-30min,离心取上清液或使用即可,巯基为产品激活剂。缓冲液配制步骤:称取  L-半胱氨酸盐酸盐5.27  g,氯化钠23.4g,加水  500  mL  溶解,另取乙二胺四乙酸二钠2.23g,加水  200  mL,溶解,合并两液混匀,用  0.1mol/L  氢氧化钠溶液或  0.1  mol/L盐酸溶液调至  pH=5.5,加水定容至  1000  mL。
储存条件: 2-8℃
EC编号: 3.4.22.32
注意: 部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。

参考文献(55篇)

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[IF=9.8] Rongxin Wen et al."Structural and functional modifications of Porphyra yezoensis proteins via ultrasound-assisted enzymolysis: insights into taste enhancement and mechanisms."FOOD CHEMISTRY.2025 Nov;492:145537 29. [IF=4.8] Qi Sun et al."Regulation mechanisms of allergenic epitope sites in casein by microbial and plant-derived proteases."Food Bioscience.2024 Dec;62:105357 28. [IF=8.5] Yile Zhao et al."Deacetylated konjac glucomannan films functionalised with carboxylated cellulose nanofiber and anti-browning components for fresh-cut apple preservation."Food Packaging and Shelf Life.2024 Dec;46:101389 27. [IF=7] Qingling Wang et al."Enhancing the physical and oxidative stability of hempseed protein emulsion via comparative enzymolysis with different proteases: Interfacial properties of the adsorption layer."FOOD RESEARCH INTERNATIONAL.2025 Feb;201:115654 26. 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[IF=1.3] Suo Qishan et al."Response surface optimization of preparation parameters and evaluation of the antioxidant and antihypertensive activities of Spirulina protein hydrolysates."Journal of Oceanology and Limnology.2024 Sep;:1-14 20. [IF=8.5] Lulu Li et al."Structure, antioxidant activity, and neuroprotective effect of black soybean (Glycine max (L.) merr.) protein hydrolysates."FOOD CHEMISTRY.2024 Sep;:141390 19. [IF=11] Lixiao Fu et al."Bromelain hydrolysis and CaCl2 coordination promote the fibrillation of quinoa protein at pH 7."FOOD HYDROCOLLOIDS.2025 Feb;159:110659 18. [IF=5.2] Wanlu Liu et al."Systematic sequence characterization of enzymatic-derived soybean peptides for precision enhancement of anti-inflammatory properties."Food Bioscience".2024 May;:104292 17. [IF=8.2] Jinxiang Xie et al."A novel angiotensin I-converting enzyme inhibitory peptide from walnut (Juglans sigillata) protein hydrolysates and its evaluation in Ang II-induced HUVECs and hypertensive rats."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES".2024 16. [IF=5.223] Peng Zhang et al."Novel hemoglobin-derived xanthine oxidase inhibitory peptides: Enzymatic preparation and inhibition mechanisms."Journal of Functional Foods.2023 Mar;102:105459 15. 刘培基,崔文甲,王文亮,弓志青,杨正友.美拉德反应改善香菇柄酶解液的风味[J].食品与发酵工业,2020,46(22):71-78. 14. 罗梦婷 石飞虹 何晨怡 等. 响应面优化蛋清蛋白抗氧化肽的酶解制备条件[J]. 浙江树人大学学报(自然科学版) 2018 18(03):22-27. 13. 吴雄, 夏建开, 苗艳丽,等. 星点设计-效应面法优化复合酶解酪蛋白制备抗凝血肽的工艺研究[J]. 药学研究, 2019, 038(006):P.336-342. 12. 向泽敏, 邱晓挺, 娄永江,等. 鲣鳔蛋白抗氧化酶解物制备工艺[J]. 水产学报, 2017, 41(006):962-970. 11. 沈文凤, 王月明, 徐志祥,等. 香菇调味料酶解工艺研究[J]. 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摩尔浓度计算器

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