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丁酰胆碱酯酶

    
BR,10 units/mg protein

BUCHE

源叶
S10162 一键复制产品信息
9001-08-5
MFCD00081455
货号 规格 价格 上海 北京 珠海 重庆 武汉 购买数量
S10162-100u
BR,10 units/mg protein

¥400.00

3 2 - - -
S10162-500u
BR,10 units/mg protein

¥1600.00

5 1 - - -
S10162-1KU
BR,10 units/mg protein

¥2600.00

4 - - - -
产品介绍 参考文献(22篇) 质检证书(COA) 摩尔浓度计算器 相关产品

产品介绍

丁酰胆碱酯酶(BChE)是一种丝氨酸水解酶,结构上类似于乙酰胆碱酯酶(AChE),但具有不同的底物特异性和抑制剂敏感性。该酶是由4个相同亚基(每个110kDa)组成的四聚体糖蛋白,又称为拟胆碱酯酶(pseudocholinesterase)。它在肝脏中合成,分布于血液、神经肌肉接头突触、神经胶质细胞和白质轴突中。

丁酰胆碱酯酶(BChE)基因突变会导致呼吸衰竭、长期瘫痪和呼吸暂停。

不同于乙酰胆碱酯酶,丁酰胆碱酯酶(BChE)可高效水解较大的胆碱酯,如丁酰胆碱和苯甲酰胆碱。酶活性可被Ca2+和Mg2+激活,在pH 6.0-8.0范围内保持恒定,受到甜菜碱、尼古丁、有机磷和氨基甲酸酯抑制。

最适pH:6-8

分子量:tetramer 440 kDa。

来源:马血清。

酶活定义:一个单位将在 pH8.0,37°C 下每分钟将1.0μ 摩尔丁酰胆碱水解为胆碱和丁酸盐。使用丁酰胆碱作为底物获得的活性是使用乙酰胆碱获得的活性的约 2.5 倍。

外观: 本品采用硫酸铵分级从马血清中制备,以冻干粉形式提供。含缓冲盐的冻干粉
溶解性: 预冷水:60  U/mL
储存条件: 2-8℃
EC编号: 3.1.1.8
用途: 马血清中的丁酰胆碱酯酶已用于对氧磷抑制丁酰胆碱酯酶的微量热法研究。这种酶也用于研究胆碱能酶的合成和抑制。选择性抑制 BChE 活性可用于有机磷酸酯的检测。由于人体内 BChE 的血液级别与对毒性神经毒剂的保护程度之间存在相关性,因此其在治疗有机磷毒性方面的应用已显示出临床潜力。胆碱酯酶在阿尔茨海默病中的作用也受到关注。对选择性抑制剂的研究可能为 BChE 在健康和患病个体中角色生理作用提供更清晰的认识。本品已用于某些蔬菜水果中胆脂酶抑制剂的筛选,用于恢复认知功能和改善记忆。它也被用来开发一种丁酰胆碱酯酶和胆碱氧化酶固定化生物嗅探器检测尼古丁。尼古丁抑制 BChE 活性。BChE 活性副产物的减少反映了尼古丁的体积。
注意: 部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。

参考文献(22篇)

17. [IF=5.3] Dingping Zhao et al."Identification of Miscanthus floridulus as a promising anti-Alzheimer’s disease and antidiabetic agent through bioactivity evaluations and chemical composition analyses."Arabian Journal of Chemistry.2024 Oct;:106037 16. [IF=5.5] Xiaojie Xu et al."The property differences of essential oils extracted by two methods from Dolichos lablab flowers: Chemical composition, anti-microorganism and cholinesterase inhibition activities."Sustainable Chemistry and Pharmacy.2024 Dec;42:101843 15. [IF=7.7] Mixue Gong et al."Visual screening of butyrylcholinesterase inhibitors by a cellulose membrane biosensor with amide-bonded immobilization of butyrylcholinesterase in a solid-phase enzyme-catalyzed reaction."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECUL 14. [IF=5.6] Qi Liu et al."A deep-red hemicyanine fluorescent probe for imaging butyrylcholinesterase in living cells and in mice with APAP-induced liver injury."TALANTA.2025 Jan;:127478 13. [IF=5.7] Yajie Zhang et al."A miniaturized colorimetric-photoacoustic dual-mode device for point-of-care testing of ascorbic acid and ascorbate oxidase."ANALYTICA CHIMICA ACTA.2025 Mar;1341:343685 12. [IF=5.3] Zhang Yue et al."Ratiometric fluorescence and colorimetric strategies for assessing activity of butyrylcholinesterase in human serum using g-C3N4 nanosheets, silver ion and o-phenylenediamine."MICROCHIMICA ACTA.2024 Jul;191(7):1-9 11. [IF=7.3] Lei Wang et al."Butyrylcholinesterase-Activated Near-Infrared Fluorogenic Probe for In Vivo Theranostics of Alzheimer’s Disease."JOURNAL OF MEDICINAL CHEMISTRY".2024;XXXX(XXX):XXX-XXX 10. [IF=1.813] Liang Liu et al."Phytochemical Profiles and Bioactivities of Parnassia palustris L.."European Journal of Integrative Medicine.2022 Nov;:102207 9. [IF=6.911] Wen-da Zhang et al."A far-red/near-infrared fluorescence probe with large Stokes shift for monitoring butyrylcholinesterase (BChE) in living cells and in vivo."ANALYTICA CHIMICA ACTA.2022 Dec;1235:340540 8. [IF=3.889] Shuang Miao et al."Aaptamine – a dual acetyl – and butyrylcholinesterase inhibitor as potential anti-Alzheimer’s disease agent."PHARMACEUTICAL BIOLOGY 7. [IF=9.231] Xiaowei Mu et al."Branched poly(ethylenimine) carbon dots-MnO2 nanosheets based fluorescent sensory system for sensing of malachite green in fish samples."FOOD CHEMISTRY.2022 Nov;394:133517 6. [IF=6.514] Bohang Zhou et al."Simple analogues of natural product chelerythrine: Discovery of a novel anticholinesterase 2-phenylisoquinolin-2-ium scaffold with excellent potency against acetylcholinesterase."Eur J Med Chem. 2020 Aug;200:112415 5. [IF=7.46] Yue Zhang et al."A dual-signal sensing platform based on nanosheet materials for ratiometric fluorescence and colorimetric detection of enzyme activities in human blood."Sensor Actuat B-Chem. 2021 Nov;346:130531 4. [IF=4.379] Zhou Bohang et al."New 2-Aryl-9-methyl-β-carbolinium salts as Potential Acetylcholinesterase Inhibitor agents: Synthesis, Bioactivity and Structure–Activity Relationship."Sci Rep-Uk. 2018 Jan;8(1):1-18 3. [IF=6.543] Zhang Jing et al."Studies of the Anti-amnesic Effects and Mechanisms of Single and Combined Use of Donepezil and Ginkgo Ketoester Tablet on Scopolamine-Induced Memory Impairment in Mice."Oxid Med Cell Longev. 2019;2019:8636835 2. 郝雅茹,李亭,张薇,李书国.基于石墨烯的丁酰胆碱酯酶传感器法快速测定水产品中的孔雀石绿[J].现代食品科技,2020,36(12):267-273. 1. 王苗苗, 严欢, 田合,等. 薰衣草提取物的体外抗氧化活性及酶抑制活性研究[J]. 食品工业科技, 2019, 40(16).

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摩尔浓度计算器

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