甘油脱氢酶

产品介绍

Stabilizer : Bovine serum albumin

Isoelectric point : 4.4±0.1

Molecular weight：approx. 390,000

Structure : 10 subunits (42,000) per enzyme molecule

Inhibitors : p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal ions (Co＋＋, Ni＋＋, Cu＋＋, Zn＋＋, Cd＋＋)

Optimum pH : 10.0－10.5

Optimum temperature : 50℃

pH Stability : pH 7.5－10.5 (25℃, 20hr)

Thermal stability : below 55℃ (pH 7.5, 15min)

Substrate specificity : This enzyme has the highest specificity for glycerol and 1,2- propanediol, and also oxidizes glycerol-α-monochlorohydrin, ethylene glycol and 2,3-butanediol . The oxidative reaction is stimulated by K＋, NH＋4 and Rb＋.

活力定义：One unit causes the formation of one micromole of NADH per minute under the conditions pH10.5,25℃.

用途：This enzyme is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis.