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β-半乳糖苷酶

    
BR,50u/mg 米曲霉

GAL

源叶
S27128 一键复制产品信息
9031-11-2
MFCD00130623
乳糖酶;β-D-半乳糖苷酶;乳糖分解酵素
β-半乳糖苷酶(9031-11-2)
货号 规格 价格 上海 北京 珠海 重庆 武汉 购买数量
S27128-25ku
BR,50u/mg 米曲霉

¥420.00

>10 9 6 - 2
S27128-100ku
BR,50u/mg 米曲霉

¥1260.00

3 3 2 - -

产品介绍

酶活定义:每个单位乳糖酶为在pH4.5,55℃下每分钟分解oNPG产生1 µmol的oNP(邻硝基酚)所需要的酶量。

温度范围:5℃~65℃,最佳温度为55℃~60℃         

pH范围:有效pH为3.0~8.0,最佳pH为4.0~5.5                                          

产品外观:白色到浅棕色粉末,颜色随批次的不同而不同。

 

产品气味:轻微的发酵气味               

SMILES: O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O)O)O
熔点: −20℃
外观: 白色或淡黄色粉末,米曲霉提取。无臭,微甜。
溶解性: 推荐使用缓冲液:  pH  4.5磷酸盐缓冲液:称取22.5g三水磷酸氢二钾、11.2g一水合柠檬酸溶解在900  mL的水中,使用1  mol/L盐酸或氢氧化钠溶液,调节pH至4.50士0.05,用去离子水定容至1  000  mL,摇匀。
储存条件: 2-8℃
EC编号: 3.2.1.23
用途: 本品仅供科研,不得用于其它用途。(以下用途仅供参考)一种能把乳糖水解成葡萄糖和半乳糖的酶。一般是指能将β-半乳糖苷水解为半乳糖和糖苷的酶;也用作酶免分析的标记酶
密度: 1
注意: 部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。

参考文献(25篇)

20. [IF=8.3] Jing-Bo Wang et al."Fluorogenic Labeling Probe for the Imaging of Endogenous β-Galactosidase Activity in Cancer and Senescent Cells."ACS Applied Materials & Interfaces.2024;16(50):68918–68927 19. [IF=7.7] Fan Xie et al."Enzymatic degalactosylation of Tamarind seed polysaccharide and its impact on the quality of frozen grass carp surimi gels."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES.2025 Feb;:140543 18. [IF=7] Hangyu Hu et al."Synthesis of novel lactose-derived oligosaccharides by two-step enzymatic reactions and structural characterization of the oligomers."FOOD RESEARCH INTERNATIONAL.2025 May;208:116177 17. [IF=10.7] Mengjiao Li et al."Enhanced electrochemiluminescence of mixed-ligand metal-organic framework with suppressed non-radiative transitions for “signal-off” biosensing of β-galactosidase."BIOSENSORS & BIOELECTRONICS.2025 Apr;:117470 16. [IF=4.8] Wenqi Shang et al."Green preparation and isolation of carrageenan oligosaccharides and assessment of their ability to inhibit melanogenesis."Food Bioscience.2024 Dec;62:105392 15. [IF=8.5] Yi Fan et al."Membrane separation assisted colorimetric/fluorescent detection of β-galactosidase-positive bacteria in milk and milk powder based on the oxidase-like activity of CoOOH nanosheets."FOOD CHEMISTRY.2024 Aug;:140946 14. [IF=10] Bo He et al."An Endocellulase-triggered NO Targeted-Release Enzyme-Prodrug Therapy System and Its Application in Ischemia Injury."Advanced Healthcare Materials.2024 Jun;:2401599 13. [IF=5.3] Zhixi Li et al."Molecular insights into the interaction between graphene oxide and β-galactosidase: From multispectral experiment, enzyme kinetics experiment to computational simulations."JOURNAL OF MOLECULAR LIQUIDS.2024 Sep;:126116 12. [IF=4.7] Xiaofeng Zeng et al."Catalytic Mode and Product Specificity of an α-Agarase Reveal Its Direct Catalysis for the Production of Agarooligosaccharides."Foods.2024 Jan;13(15):2351 11. [IF=5.2] Yaozu Guo et al."Increase in Lactulose Content in a Hot-Alkaline-Based System through Fermentation with a Selected Lactic Acid Bacteria Strain Followed by the β-Galactosidase Catalysis Process."Foods.2023 Jan;12(23):4317 10. [IF=5.6] Xu Wencheng et al."Tetrandrine inhibits the proliferation of mesangial cells induced by enzymatically deglycosylated human IgA1 via IgA receptor/MAPK/NF-κB signaling pathway."Frontiers in Pharmacology.2023 Jun;14: 9. [IF=4.125] Xiaolong Ren et al."Effects of tamarind seed polysaccharides on physicochemical characteristics of frozen dough: structure–function relationship."JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE.2023 May;: 8. [IF=5.561] Yan Yang et al."Optimization of Extraction Process of Polysaccharides MAP-2 from Opuntia Milpa Alta by Response Surface Methodology and Evaluation of Its Potential as α-Glucosidase Inhibitor."Foods.2022 Jan;11(21):3530 7. [IF=6.558] Menglan Shi et al."Ratiometric fluorescence monitoring of α-glucosidase activity based on oxidase-like property of MnO2 nanosheet and its application for inhibitor screening."Anal Chim Acta. 2019 Oct;1077:225 6. Li, Ai-li, et al. "Effect of 2′-fucosyllactose supplementation on intestinal flora in mice with intestinal inflammatory diseases." International Dairy Journal 110 (2020): 104797.https://doi.org/10.1016/j.idairyj.2020.104797 5. Deng, Ziyu, et al. "Carboxymethylpachymaran entrapped plant-based hollow microcapsules for delivery and stabilization of β-galactosidase." Food & function 10.8 (2019): 4782-4791.https://doi.org/10.1039/C9FO00649D 4. Deng, Ziyu, et al. "Carboxymethylpachymaran-zein coated plant microcapsules-based β-galactosidase encapsulation system for long-term effective delivery." Food Research International 128 (2020): 108867.https://doi.org/10.1016/j.foodres.2019.108867 3. Li, Yuan, et al. "Metal–Enzyme Hybrid Microspheres Assembled via Mg2+-Allosteric Effector." Industrial & Engineering Chemistry Research 59.46 (2020): 20278-20284.https://doi.org/10.1021/acs.iecr.0c04721 2. Ziyu Deng, Jing Li, Yaqiong Pei, Jiawei Wan, Bin Li, Hongshan Liang, Oligosaccharides act as the high efficiency stabilizer for β-galactosidase under heat treatment, International Journal of Biological Macromolecules, Volume 137, 2019, Pages 69-76, ISSN 01 1. 罗艳华 王全杰 陈沛海 等. 乌氏粘度计测定废革屑水解液粘均分子量的研究[J]. 皮革与化工 2017 01(v.34;No.171):14-18.

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