木瓜蛋白酶 ≥2000units/mg,以酪蛋白为底物
产品介绍
酶活定义: 1g 固体酶(或 1mL 液体酶),在温度 37℃、pH7.0 条件下,1min 水解酪蛋白底物产生相当于 1µg 酪 氨酸的酶量,为 1 个酶活力单位,以“U/g( 或 U/mL)”表示。反应体系建议加入终浓度5-10mM的L-半胱氨酸盐酸盐作为激活剂。
分子量:21000~23000
最适温度:37~60℃
最适pH:5.5~7.5
性状:为乳白色至微黄色粉末
成分:木瓜蛋白酶,葡萄糖(含有1%以下的凝乳酶)
| 外观: | 白色至米黄色粉末 |
|---|---|
| 溶解性: | L-半胱氨酸盐酸盐缓冲液配制,巯基为产品激活剂。缓冲液配制步骤:称取 L-半胱氨酸盐酸盐5.27 g,氯化钠23.4g,加水 500 mL 溶解,另取乙二胺四乙酸二钠2.23g,加水 200 mL,溶解,合并两液混匀,用 0.1mol/L 氢氧化钠溶液或 0.1 mol/L盐酸溶液调至 pH=7.0,加水定容至 1000 mL。 |
| 储存条件: | 2-8℃ |
| EC编号: | 3.4.22.2 |
| 用途: | 一种能水解多肽、酰胺和酯的酶。其分子由212残基的折叠多肽键组成。微有吸湿性。对光敏感。不完全的溶于水和甘油,几乎不溶于多数有机溶剂。酶反应:裂解Arg-、Lys-、Phe-、X氨基酸。模型底物为苯甲酰精氨酸乙酯(BAEE)、N-苯甲酰-L-精氨酸对硝基苯胺(N-α-Bz-L-Arg-4-NA)、苄氧羰酰缬氨酰甘氨酰精氨酸对硝基苯胺(Chromozym TRY)。激活剂有半胱氨酸(Cys)、谷胱甘肽(GSH)、EDTA、还原剂、氰化物。抑制剂有汞(Hg2+)、其他重金属、碘乙酸、N-对基顺丁烯二 |
| 注意: | 部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。 |
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