货号	规格	价格	上海	北京	珠海	重庆	武汉
S80190-1mg买3赠1	99.80%	￥160.00 S80190-1mg ￥160.00 现货（买3赠1）	1 1	-	-	-	-
S80190-2mg买3赠1	99.80%	￥288.00 S80190-2mg ￥288.00 现货（买3赠1）	4 4	-	-	-	-
S80190-5mg买3赠1	99.80%	￥392.00 S80190-5mg ￥392.00 现货（买3赠1）	5 5	-	-	-	-
S80190-10mg买3赠1	99.80%	￥712.00 S80190-10mg ￥712.00 现货（买3赠1）	1 1	-	-	-	-
S80190-25mg买3赠1	99.80%	￥1440.00 S80190-25mg ￥1440.00 现货（买3赠1）	3 3	-	-	-	-
S80190-50mg买3赠1	99.80%	￥2560.00 S80190-50mg ￥2560.00 现货（买3赠1）	6 0 6	-	-	-	-
S80190-100mg买3赠1	≥99%	￥3750.00 S80190-100mg ￥3750.00 2-3天（买3赠1）	0 货期：2-3天	-	-	-	-

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产品描述：

Thiazovivin, a ROCK inhibitor (IC50: 0.5 μM), increases the survival rate of hESC.

靶点：

ROCK

细胞实验：

Chemically defined and feeder-free human embryonic stem cell (hESC) culture was described briefly as following. hESCs were grown on Matrigel-coated tissue culture plates in N2B27-CDM [DMEM-F12 supplemented with 1× N2 supplements, 1× B27 supplements, 2 mM L glutamine, 0.11 mM 2-mercaptoethanol, 1× nonessential amino acids, and 0.5 mg/mL BSA (fraction V)] and 20 ng/mL bFGF. Human ESCs were passaged every five to six days with 0.05% trypsin. Murine ESCs are cultured in knockout DMEM supplement with 2 mM L glutamine, 1× nonessential amino acids, 15% serum replacement, and 1 × 10^3 ng/mL leukemia inhibitory factor (LIF). For clonal survival assays, single hESCs were diluted to clonal density and plated onto 96-well Matrigel-coated plate. For low-density survival assays, 500 cells were plated onto 96-well Matrigel-coated plate. To visualize hESC colonies, cultures were fixed in 4% paraformaldehyde in phosphate buffered saline (PBS) for 5 min, washed once in PBS, then stained for alkaline phosphatase (ALP) activity

参考文献：

1.Park S, et al. Thiazovivin, a Rho kinase inhibitor, improves stemness maintenance of embryo-derived stem-like cells under chemically defined culture conditions in cattle. Anim Reprod Sci. 2015 Oct;161:47-57. 2.Hu K, et al. Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cells. Blood. 2011 Apr 7;117(14):e109-19. 3.Xu Y, et al. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci U S A. 2010 May 4;107(18):8129-34.

溶解性：

DMSO:31.1 mg/mL (100 mM)

保存条件：

-20℃

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	3.212 ml	16.059 ml	32.117 ml
5 mM	0.642 ml	3.212 ml	6.423 ml
10 mM	0.321 ml	1.606 ml	3.212 ml
50 mM	0.064 ml	0.321 ml	0.642 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

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