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产品描述： Ciclesonide(RPR251526) is a glucocorticoid used for the treatment of obstructive airway diseases. |
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靶点：
Glucocorticoid Receptor;GlucocorticoidReceptor |
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细胞实验：
Metabolism of ciclesonide: After initial cultivation, A549 cells are washed five times with 10 ml/dish sterile PBS. An ethanolic stock solution of ciclesonide is diluted 1000-fold with assay medium to generate the initial ciclesonide concentration. The cells are incubated for 1 h in DMEM/0.1% (w/v) BSA (5 ml/dish) containing 0.02 μM ciclesonide as described above in a CO2 incubator. At the end of the incubation period, dishes are washed five times with PBS and incubated for 3, 6, or 24 h at the same conditions in 5 ml/dish fresh medium (DMEM/0.1% (w/v) BSA). At each indicated time point, the medium is removed by aspiration, 5 mM EDTA-4Na/PBS added and dishes are incubated for further 5 min. The cells are detached from the dishes and harvested. Each dish is washed five times with icechilled PBS. (Only for Reference) |
参考文献：
1.Nonaka T, et al. BMC Pharmacol. 2007, 7:12. 2.Guo Z, et al. Am J Ther. 2006, 13(6):490-501. |
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溶解性：
DMSO:54.1 mg/mL (100 mM) Ethanol:54.1 mg/mL (100 mM) |
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保存条件：
2-8℃ |
配置溶液浓度参考：
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1mg |
5mg |
10mg |
| 1 mM |
1.849 ml |
9.247 ml |
18.495 ml |
| 5 mM |
0.37 ml |
1.849 ml |
3.699 ml |
| 10 mM |
0.185 ml |
0.925 ml |
1.849 ml |
| 50 mM |
0.037 ml |
0.185 ml |
0.37 ml |
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| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
北京