| 产品描述: | Biotin-VAD-FMK is a cell permeable, irreversible biotin-labeled caspase inhibitor, used to identify active caspases in cell lysates. |
| 靶点: |
Caspase |
| 体外研究: |
Biotin-VAD-FMK is a synthetic peptide designed as a methyl ester to facilitate cell permeability. |
| 体内研究: |
To examine whether caspase-2 is activated in the absence of proteolytic cleavage, an in vivo affinity labeling approach is used, using the biotinylated caspase inhibitor biotin-VAD-fmk that detects only active caspases |
| 参考文献: |
1. Li J, et al. Nitric oxide suppresses apoptosis via interrupting caspase activation and mitochondrial dysfunction in cultured hepatocytes. J Biol Chem. 1999 Jun 11;274(24):17325-33. 2. Samraj AK, et al. Loss of caspase-9 reveals its essential role for caspase-2 activation and mitochondrial membranedepolarization. Mol Biol Cell. 2007 Jan;18(1):84-93. |
| 溶解性: |
soluble in DMSO |
| 保存条件: |
-20℃ |
| 配置溶液浓度参考: |
|
1mg |
5mg |
10mg |
| 1 mM |
1.486 ml |
7.432 ml |
14.863 ml |
| 5 mM |
0.297 ml |
1.486 ml |
2.973 ml |
| 10 mM |
0.149 ml |
0.743 ml |
1.486 ml |
| 50 mM |
0.03 ml |
0.149 ml |
0.297 ml |
|
| 注意: |
部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。 |