货号	规格	价格	上海	北京	珠海	重庆	武汉	购买数量
S83624-5mg	≥98%	￥2580.00 S83624-5mg ￥2580.00 3-5天	0 货期：3-5天	-	-	-	-
S83624-10mg	≥98%	￥4180.00 S83624-10mg ￥4180.00 3-5天	0 货期：3-5天	-	-	-	-

货号

规格

价格

上海

北京

珠海

重庆

武汉

购买数量

S83624-5mg

≥98%

￥2580.00

货期：3-5天

S83624-10mg

≥98%

￥4180.00

货期：3-5天

产品描述：

Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA.

靶点：

Others

体外研究：

The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similar excitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around an emission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethylformamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage, the solutions are instead frozen at ≤-20 °C. The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine and thymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells.

参考文献：

1. a b c 'Hoechst Stains'. Invitrogren (Molecular Probes). 2. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505. 3. Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68.

溶解性：

DMSO

保存条件：

-20℃

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	1.871 ml	9.356 ml	18.713 ml
5 mM	0.374 ml	1.871 ml	3.743 ml
10 mM	0.187 ml	0.936 ml	1.871 ml
50 mM	0.037 ml	0.187 ml	0.374 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

质检证书(COA)

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请输入货号和一个与之匹配的批号。
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批号：JS298415 货号：S20001-25g
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摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

质量

浓度

体积

分子摩尔量

计算重置

meta-iodoHoechst 33258