| 货号 | 规格 | 价格 | 上海 | 北京 | 珠海 | 重庆 | 武汉 | 购买数量 |
|---|---|---|---|---|---|---|---|---|
S85156-1mg买3赠1 |
99.80% | ￥236.00 |
5 | - | - | - | - |
|
S85156-5mg买3赠1 |
99.80% | ￥370.00 |
6 | - | - | - | - |
|
S85156-10mg买3赠1 |
99.80% | ￥570.00 |
4 | - | - | - | - |
|
S85156-25mg买3赠1 |
99.80% | ￥1330.00 |
6 | - | - | - | - |
|
S85156-50mg买3赠1 |
≥99% | ￥2220.00 |
货期：2-3天 | - | - | - | - |
|
S85156-100mg买3赠1 |
≥99% | ￥3260.00 |
货期：2-3天 | - | - | - | - |
|
| 产品描述： | Madrasin (DDD00107587) is a splicing inhibitor that prevents formation of both splicing intermediates and products in vitro and interferes with one or more early steps in the pathway of spliceosome assembly. Madrasin also can inhibit pre-mRNA splicing in vitro and modify splicing of endogenous pre-mRNA in cells | ||||||||||||||||||||
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| 靶点： | pre-mRNA splicing;DNA/RNASynthesis | ||||||||||||||||||||
| 体外研究： | Madrasin (10-30 μM; 4-24 hours; HeLa cells) treatment inhibits the splicing of each of the RIOK3, BRD2, and Hsp40, MCL1, CCNA2, AURKA and p27 pre-mRNAs in both HeLa and HEK293 cells. Madrasin (10-30 μM; 4-24 hours; HeLa and HEK293 cells) treatment shows a dose- and time-dependent inhibitory effect on cell cycle progression. RT-PCR Cell Line: HeLa cells Concentration: 10 μM, 20 μM, or 30 μM Incubation Time: 4 hours, 8 hours, or 24 hours Result: Increased in inhibition of pre-mRNA splicing. Cell Cycle Analysis Cell Line: HeLa and HEK293 cells Concentration: 10 μM, 20 μM, or 30 μM Incubation Time: 4 hours, 8 hours, or 24 hours Result: In the presence of 10 μM 8 hours after treatment, the proportion of cells in G2, M, and S phases increased, with a concomitant decrease in the number of G1 phase cells. This effect increased over time, with >40% of cells in G2 and M phase and >50% in S phase 24 hours. | ||||||||||||||||||||
| 参考文献： | 1. Andrea Pawellek et al. Identification of Small Molecule Inhibitors of Pre-mRNA Splicing. J Biol Chem, 2014 Dec 12, 289(50): 34683-34698. | ||||||||||||||||||||
| 溶解性： | Soluble in DMSO | ||||||||||||||||||||
| 保存条件： | -20℃ | ||||||||||||||||||||
| 配置溶液浓度参考： |
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| 注意： | 部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
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