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产品描述： PHTPP is a selective ERβ antagonist. |
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靶点：
Estrogen Receptor/ERR;Estrogen/progestogen Receptor;EstrogenReceptor/ERR;Estrogen/progestogenReceptor |
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细胞实验：
Cells are plated at a density of 3 to 4×10^5 in 1 mL of medium per well in a 24-well plate for cell viability and cAMP analysis. Cells are cultured in Dulbecco's Modified Eagle Medium with PHTPP (1 μM), or ethanol (0.1%) as the vehicle. The incubator is set to an atmosphere of 5% CO2 in air at 37°C, and cultures are allowed to acclimate for at least 24 h. The culture medium is then aspirated and replaced with serum-free DMEM/F12 containing 0.1 μM androstenedione. The cells are collected for intracellular cAMP and to test cell viability. They are for reference only. |
参考文献：
1.Wang K, Chen Y, Gao S, et al. Norlichexanthone purified from plant endophyte prevents postmenopausal osteoporosis by targeting ERα to inhibit RANKL signaling[J]. Acta Pharmaceutica Sinica B. 2020 2.Compton DR, etal. Pyrazolo[1,5-a]pyrimidines: estrogen receptor ligands possessing estrogen receptor beta antagonist activity. J Med Chem. 2004 Nov 18;47(24):5872-93. |
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溶解性：
Soluble in DMSO |
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保存条件：
2-8℃ |
配置溶液浓度参考：
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1mg |
5mg |
10mg |
| 1 mM |
2.362 ml |
11.812 ml |
23.623 ml |
| 5 mM |
0.472 ml |
2.362 ml |
4.725 ml |
| 10 mM |
0.236 ml |
1.181 ml |
2.362 ml |
| 50 mM |
0.047 ml |
0.236 ml |
0.472 ml |
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| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
北京