| 货号 | 规格 | 价格 | 上海 | 北京 | 珠海 | 重庆 | 武汉 | 购买数量 |
|---|---|---|---|---|---|---|---|---|
S87651-5mg促销 |
99.40% | ￥980.00 ￥500.00 |
1 | - | - | - | - |
|
S87651-10mg促销 |
99.40% | ￥1500.00 ￥700.00 |
2 | - | - | - | - |
|
S87651-25mg促销 |
99.40% | ￥2900.00 ￥1380.00 |
2 | 2 | 1 | - | - |
|
S87651-100mg促销 |
99.40% | ￥5800.00 ￥2900.00 |
6 | - | - | - | - |
|
S87651-1g |
≥99% | ￥6500.00 |
货期：2-3天 | - | - | - | - |
|
| 产品描述： | D-Lin-MC3-DMA, an ionizable cationic lipid, is a potent siRNA delivery vehicle. | ||||||||||||||||||||
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| 体外研究： | Replacement of DSPC by unsaturated PCs and DLin-MC3-DMA by the related lipid DLin-KC2-DMA resulted in highly potent transfection reagents for HeLa cells in vitro | ||||||||||||||||||||
| 体内研究： | 含有二硬脂酰磷脂酰胆碱 (DSPC) 和可电离氨基脂质（如二亚油基甲基-4-二甲氨基丁酸酯 (DLin-MC3-DMA)）的脂质纳米颗粒 (LNP) 是有效的体内 siRNA 递送载体。LNP-siRNA 系统经过优化，可在小鼠静脉注射后在肝细胞中实现最大的基因沉默效力，其中包含 DLin-MC3-DMA (MC3)、DSPC、胆固醇和聚乙二醇 (PEG)-脂质，摩尔比为 50/10/38.5/1.5。DLin-MC3-DMA 表现出良好的的 pKa 值，可显著提高效力。 | ||||||||||||||||||||
| 细胞实验： | White leghorn chicken embryos at stage 24 (30) were removed and dissected in Hanks buffered saline solution (HBSS).?Forelimbs were pooled and treated for 1 h in dispase to remove epithelial ectoderm.?Limb mesenchyme was dissociated, and resuspended in DMEM with 5% FBS and 1% penicillin/streptomycin.?Cells were plated onto 96-well plates at a density of 2x10^5 or 2x10^7 cells/ml and treated with 0-40 μg/ml LNP-pDNA.?Cultures were incubated overnight at 37℃ and resuspended in PBS buffer for expression analysis using a BD LSRII flow cytometer.?Cell survival was assessed in low-density cultures after 24 h of treatment, by counting the adherent cells remaining after PBS washes.?Viability was determined based on average counts of all adherent cells within a single field of view (100x) normalized to cell counts of untreated cultures. | ||||||||||||||||||||
| 参考文献： | 1. Kulkarni J A , Myhre J L , Chen S , et al. Design of lipid nanoparticles for, in vitro, and, in vivo, delivery of plasmid DNA[J]. Nanomedicine: Nanotechnology, Biology and Medicine, 2017, 13(4):1377-1387. | ||||||||||||||||||||
| 溶解性： | Soluble in DMSO、Ethanol | ||||||||||||||||||||
| 保存条件： | -20℃ | ||||||||||||||||||||
| 配置溶液浓度参考： |
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| 注意： | 部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
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危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京