货号	规格	价格	上海	北京	珠海	重庆	武汉
S88548-5mg买3赠1	95.00%	￥400.00 S88548-5mg ￥400.00 现货（买3赠1）	7 7	-	-	-	-
S88548-10mg买3赠1	95.00%	￥600.00 ￥540.00 S88548-10mg ￥540.00 现货（买3赠1）	8 8	-	-	-	-
S88548-25mg买3赠1	95.00%	￥990.00 S88548-25mg ￥990.00 现货（买3赠1）	6 6	-	-	-	-
S88548-100mg买3赠1	95.00%	￥2250.00 ￥2020.00 S88548-100mg ￥2020.00 现货（买3赠1）	7 7	-	-	-	-

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产品描述：

INH14 is a novel inhibitor of the IKKα/β-dependent TLR inflammatory response (IC50s: 8.97/3.59 μM for IKKα/IKKβ).

靶点：

IKKα:8.97 μM (cell free), IKKβ:3.59 μM (cell free);IκB/IKK

体外研究：

Overexpression of proteins that are part of the TLR2 pathway in cells treated with INH14 indicated that the target lay downstream of the complex TAK1/TAB1. INH14 decreased IkBα degradation in cells activated by lipopeptide (TLR2 ligand). The kinases IKKα and/or IKKβ were the targets of INH14, which was confirmed with kinase assays (IC50 IKKα=8.97μM; IC50 IKKβ=3.59μM).

体内研究：

In vivo experiments showed that INH14 decreased TNFα formed after lipopeptide-induced inflammation, and treatment of ovarian cancer cells with INH14 led to a reduction of NF-kB constitutive activity and a reduction in the wound-closing ability of these cells.

细胞实验：

Human primary monocytes (8 x 10^4 cells/well) were seeded and incubated overnight with the compound, media control, or SDS (0.02%). Then, the tetrazolium salt WST-8 was added, and the cells were incubated for one additional hour at 37°C. During this period, the dehydrogenase activity of viable cells led to the production of the coloured product (formazan). The cell viability was measured with a Victor plate reader as an increase in the absorbance at 450 nm.

动物实验：

8-week old, male, pathogen-free C57BL/6J mice were maintained at the animal facility (12 h light/dark cycle; standard rodent chow and water available ad libitum). For lipopeptide-induced inflammation, 5 μg/g of INH14 or vehicle were administered intraperitoneally. After one hour, P2 (2.5 μg/g) was injected and 25 μl of tail vein blood were collected at that time point (0 h) and 2 h after. The blood was centrifuged at 5 x 10^3 x g, and the supernatant frozen at -20 °C until further cytokine measurement by ELISA.

参考文献：

1. Drexel M, et al. INH14, a Small-Molecule Urea Derivative, Inhibits the IKKα/β-Dependent TLR Inflammatory Response. Chembiochem. 2019 Mar 1;20(5):710-717.

溶解性：

soluble in DMSO

保存条件：

-20°C

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	4.161 ml	20.807 ml	41.615 ml
5 mM	0.832 ml	4.161 ml	8.323 ml
10 mM	0.416 ml	2.081 ml	4.161 ml
50 mM	0.083 ml	0.416 ml	0.832 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

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摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

质量

浓度

体积

分子摩尔量

计算重置

INH14

1-(4-ethylphenyl)-3-phenylurea

参考文献

质检证书(COA)

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摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)