货号	规格	价格	上海	北京	珠海	重庆	武汉
S88666-2mg买3赠1	99.90%	￥210.00 S88666-2mg ￥210.00 现货（买3赠1）	503 2 >10	2	-	-	-
S88666-5mg买3赠1	99.90%	￥315.00 S88666-5mg ￥315.00 现货（买3赠1）	206 >10	-	-	-	-
S88666-10mg买3赠1	99.90%	￥600.00 S88666-10mg ￥600.00 现货（买3赠1）	104 2 >10	2	-	-	-
S88666-25mg买3赠1	99.90%	￥1480.00 S88666-25mg ￥1480.00 现货（买3赠1）	46 >10	-	-	-	-
S88666-50mg买3赠1	99.90%	￥1790.00 S88666-50mg ￥1790.00 现货（买3赠1）	24 >10	-	-	-	-
S88666-100mg买3赠1	99.90%	￥3400.00 S88666-100mg ￥3400.00 现货（买3赠1）	15 >10	-	-	-	-

大包装询价收藏产品

加入购物车

产品描述：

NSC 185058 is an inhibitor of ATG4B, a major cysteine protease. It also can attenuate the autophagic activity

靶点：

Autophagy; Cysteine Protease;CysteineProtease; Autophagy

体外研究：

NSC185058 effectively suppressed starvation-induced autophagy in both cell types. GFP-LC3B labeled AVs were absent in starved MDA-MB468 cells when treated with NSC185058. The inhibitory effects of both NSC185058 on starvation-induced protein turnover were comparable to 3MA.

体内研究：

Mice were injected at 2 24 h intervals with vehicle or NSC185058, autophagy induced by 20 h fasting. Fasting resulted in an increase of GFP-LC3B AVs compared with ad libitum fed mice. NSC185058 significantly decreased the appearance of GFP-LC3B AVs compared with fasted mice

细胞实验：

Saos-2 transiently expressing FYVE-RFP was incubated in DMEM medium supplemented with amino acids and serum (Fed) or Krebs-Heinseleit medium lacking amino acids and serum (Starved). The starved cells were incubated in the presence and absence of the NSC185058 or NSC377071 for 4 h at which time the cells were examined by fluorescence microscopy. The numbers of red 'dots' per cell was quantified using ImageJ software. Taking into account basal responses, those cells with 3 or more puncta were defined as PtdIns3K active

动物实验：

Immunodeficient nu/nu nude female mice were injected subcutaneously with 6 × 10^6 Saos-2 (GFP-LC3B), shCon-Saos-2(GFP-LC3B) or shATG4B-Saos-2(GFP-LC3B) cells. Palpable Saos-2 (GFP-LC3B) tumors developed in 7 to 10 d, at which time, the mice were divided into 2 groups and injected IP on Monday, Wednesday, and Friday with either peanut oil vehicle or NSC185058 (100 mg/kg body weight) dissolved in peanut oil. Over time, the tumor dimensions were measured using calipers and volumes calculated using the formula: (W2 × L)/2.

参考文献：

1. Akin D, et al. A novel ATG4B antagonist inhibits autophagy and has a negative impact on osteosarcoma tumors. Autophagy. 2014;10(11):2021-35.

溶解性：

Soluble in DMSO、Ethanol

保存条件：

2-8°C

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	4.645 ml	23.226 ml	46.452 ml
5 mM	0.929 ml	4.645 ml	9.29 ml
10 mM	0.465 ml	2.323 ml	4.645 ml
50 mM	0.093 ml	0.465 ml	0.929 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

质检证书(COA)

输入产品批号：

如何获取质检证书(COA)?

请输入货号和一个与之匹配的批号。
例如:
批号：JS298415 货号：S20001-25g
在货品标签上如何找到货号和批号?

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

质量

浓度

体积

分子摩尔量

计算重置

NSC 185058

2-Pyridinecarbothioamide, N-2-pyridinyl-

参考文献

质检证书(COA)

如何获取质检证书(COA)?

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)