货号	规格	价格	上海	北京	珠海	重庆	武汉
S89118-5mg买3赠1	99.70%	￥560.00 S89118-5mg ￥560.00 现货（买3赠1）	5 5	-	-	-	-
S89118-10mg买3赠1	99.70%	￥880.00 S89118-10mg ￥880.00 现货（买3赠1）	6 6	-	-	-	-
S89118-25mg买3赠1	99.70%	￥1760.00 S89118-25mg ￥1760.00 现货（买3赠1）	5 5	-	-	-	-
S89118-100mg	≥98%	￥5280.00 S89118-100mg ￥5280.00 2-3天	0 货期：2-3天	-	-	-	-

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产品描述：

CU-T12-9 is a specific TLR1/2 agonist with EC50 of 52.9 nM in HEK-Blue hTLR2 SEAP assay. CU-T12-9 activates both the innate and the adaptive immune systems. CU-T12-9 selectively activates the TLR1/2 heterodimer, not TLR2/6. CU-T12-9 signals through NF-κB and invokes an elevation of the downstream effectors TNF-α, IL-10, and iNOS

靶点：

TLR2:52.9 nM (EC50, in HEK-Blue cells);TLR1:52.9 nM (EC50, in HEK-Blue cells);TLR

体外研究：

CU-T12-9 directly targets TLR1/2 to initiate downstream signaling. By binding to both TLR1 and TLR2, CU-T12-9 facilitates the TLR1/2 heterodimeric complex formation, which in turn activates the downstream signaling. CU-T12-9 activates the TLR1/2 pathway by inducing NF-κB activation to trigger downstream signaling, such as secreted embryonic alkaline phosphatase (SEAP), NO, and TNF-α[1].CU-T12-9 (0.39-100 μM; 24 hours) does not produce toxicity up to 100 μM in HEK-Blue hTLR2 and Raw 264.7 cells. CU-T12-9 up-regulates the mRNA levels of TLR1, TLR2, TNF, IL-10, and iNOS. CU-T12-9 (0.1-10 μM) activates TLR1 mRNA and iNOS mRNA after Raw 264.7 cells are treated for 24 hours. CU-T12-9 (0.1-10 μM) activates TLR2 and IL-10 mRNA after Raw 264.7 cells are treated for 2 hours. CU-T12-9 (0.1-10 μM) activates TNF mRNA after Raw 264.7 cells are treated for 8 hours. Cell Cytotoxicity Assay Cell Line: HEK-Blue hTLR2 and Raw 264.7 macrophage cells Concentration: 0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, and 100 μM Incubation Time: 24 hours Result: No toxicity was seen up to 100 μM. RT-PCR Cell Line: Raw 264.7 cells Concentration: 0.1, 1, 10 μM Incubation Time: 24 hours for TLR1 and iNOS mRNA assay2 hours for TLR2 and IL-10 mRNA assay8 hours for TNF mRNA assay Result: Triggered TLR1 mRNA and iNOS mRNA at 24 hours dose-dependently.Dose-dependent activation of TLR2 mRNA and IL-10 mRNA at 2 hours.Showed dose-dependent activation of TNF mRNA at 8 hours.

参考文献：

1. Cheng K, Gao M, Godfroy JI, Brown PN, Kastelowitz N, Yin H. Specific activation of the TLR1-TLR2 heterodimer by small-molecule agonists. Sci Adv. 2015;1(3). pii: e1400139. PubMed PMID: 26101787; PubMed Central PMCID: PMC4474499.

溶解性：

Soluble in DMSO

保存条件：

-20℃

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	2.76 ml	13.8 ml	27.601 ml
5 mM	0.552 ml	2.76 ml	5.52 ml
10 mM	0.276 ml	1.38 ml	2.76 ml
50 mM	0.055 ml	0.276 ml	0.552 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

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质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

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分子摩尔量

计算重置

CU-T12-9

N-methyl-4-nitro-2-(4-(4-(trifluoromethyl)phenyl)-1H-imidazol-1-yl)aniline

参考文献

质检证书(COA)

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摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)