货号	规格	价格	上海	北京	珠海	重庆	武汉
S89147-5mg买3赠1	98.20%	￥800.00 S89147-5mg ￥800.00 现货（买3赠1）	188 >10	-	-	-	-
S89147-10mg买3赠1	98.20%	￥1400.00 S89147-10mg ￥1400.00 现货（买3赠1）	98 >10	-	-	-	-
S89147-25mg买3赠1	98.20%	￥2800.00 S89147-25mg ￥2800.00 现货（买3赠1）	43 >10	-	-	-	-
S89147-100mg	98.20%	￥8400.00 S89147-100mg ￥8400.00 现货	17 >10	-	-	-	-

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产品描述：

NAE-IN-M22 is a potent, selective and reversible inhibitor of NEDD8 activating enzyme (NAE), with potency in micromolar range. NAE-IN-M22 inhibits multiple cancer cell lines and induces apoptosis in A549 cells. NAE-IN-M22 also can inhibit tumor growth in vivo

靶点：

NEDD8 activating enzyme (NAE);Apoptosis; E1/E2/E3Enzyme

体外研究：

M22 (0.37-90 μM; 24 h) blocks neddylation pathway selectively and suppresses degradation of CRL substrates in A549 cells. M22 (0.1-100 μM; 48 h) inhibits A549 cell proliferation completely at 30 µM (GI50=5.5 µM, GI90=19.3 µM). M22 (15-30 μM; 36 h) promotes apoptosis in A549 cell line. Cell Viability Assay Cell Line: A549 cells Concentration: 0.37, 1.11, 3.33, 10, 30, 90 μM Incubation Time: 24 hours Result: Inhibited formations of Uba3-NEDD8 and Ubc12-NEDD8.Resulted in a corresponding decrease in the abundance of Cullins-NEDD8.Decreased the degradations of p27 and CDT1.Prevented p53 from degradation.

体内研究：

M22 (60 mg/kg; i.p. once daily for 14 d) inhibits tumor growth in nude mice bearing AGS xenografts[1].M22 (0.36-90 μM) has low acute toxicity in zebrafish model

参考文献：

1. Lu P, et, al. Discovery of a novel NEDD8 Activating Enzyme Inhibitor with Piperidin-4-amine Scaffold by Structure-Based Virtual Screening. ACS Chem Biol. 2016 Jul 15;11(7):1901-7.

溶解性：

Soluble in DMSO

保存条件：

-20℃

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	2.752 ml	13.762 ml	27.523 ml
5 mM	0.55 ml	2.752 ml	5.505 ml
10 mM	0.275 ml	1.376 ml	2.752 ml
50 mM	0.055 ml	0.275 ml	0.55 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

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摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

质量

浓度

体积

分子摩尔量

计算重置

M22 NEDD8 inhibitor

参考文献

质检证书(COA)

如何获取质检证书(COA)?

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)