| 产品描述： | CTB是一种p300激活剂，CTB和p300的最低对接能量为7.72 kcal/mol |
| 靶点： |
p300;EpigeneticReaderDomain |
| 体外研究： |
CTB effectively induces apoptosis, up-regulates the expression of P300, and increases the acetylation and total P53 protein levels in MCF-7 cells |
| 体内研究： |
CTB combinated with CpG, and aluminum hydroxide playsa synergistic effect to enhance ClfA221-550-induced immune responses, which possesses the potential to develop a novel vaccine to prevent infection of S. aureus |
| 细胞实验： |
Cell lines: MCF-7 and MRC-5 cells Concentrations: 85.43 µM Incubation Time: 24, 48, 72 h Method: MCF-7 and MRC-5 are cultured in RPMI-1640 and treated with or without CTB at a concentration of 85.43 μmol/L at different times (24, 48 and 72 h). The percentage of apoptotic cells are measured by flow cytometry.Real-time quantitative RT-PCR is performed to estimate the mRNA expression of P300 in MCF-7 and MRC-5 with CTB at different times. ELISA and Bradford protein techniques are used to detect levels of total and acetylated P53 protein generated in MCF-7 and MRC-5 |
| 动物实验： |
Animal Models: Specific-pathogen-free BALB/c mice Dosages: 100 μg Administration: i.m. |
| 参考文献： |
1. Devipriya B, et al. Exploring the binding affinities of p300 enzyme activators CTPB and CTB using docking method. Indian J Biochem Biophys. 2010 Dec;47(6):364-9. 2. Dastjerdi MN, et al. The effect of CTB on P53 protein acetylation and consequence apoptosis on MCF-7 and MRC-5 cell lines. Adv Biomed Res. 2013 Mar 6;2:24. 3. Ma J, et al. The novel combinations of CTB, CpG, and aluminum hydroxide significantly enhanced the immunogenicity of clumping factor A 221-550 of Staphylococcus aureus. Biosci Biotechnol Biochem. 2020 Sep;84(9) |
| 溶解性： |
Soluble in DMSO、Ethanol |
| 保存条件： |
-20℃ |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.909 ml |
14.546 ml |
29.093 ml |
| 5 mM |
0.582 ml |
2.909 ml |
5.819 ml |
| 10 mM |
0.291 ml |
1.455 ml |
2.909 ml |
| 50 mM |
0.058 ml |
0.291 ml |
0.582 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京