| 产品描述： | AEG3482 is a potent antiapoptotic compound that inhibits Jun kinase (JNK) activity through induced expression of heat shock protein 70 (HSP70). AEG3482 directly binds HSP90, thereby facilitating HSF1-dependent expression of HSP70 and HSP25 |
| 靶点： |
JNK |
| 体外研究： |
AEG3482 (0.3-30 μM; 2 d) inhibits nerve growth factor (NGF) withdrawal-induced death in SCG neurons, with an EC50 of ∼20 μM. AEG3482 (1-80 μM; 40 h) inhibits p75NTR- and NRAGE- mediated apoptosis of PC12 cells in a dose-dependent manner. AEG3482 (10-40 μM; 30 h) inhibits p75NTR- and NRAGE-mediated JNK activation in PC12 cells. Apoptosis Analysis Cell Line: PC12 cells Concentration: 1, 2.5, 5, 10, 20, 40, 80 μM Incubation Time: 40 hours Result: Reduced p75NTR- or NRAGE-induced cell death by greater than 90% at the concentration of 40 μM.Exerted a slight toxic effect in cells infected with the LacZ control at the concentration of 80 μM. Western Blot Analysis Cell Line: PC12 cells Concentration: 10, 20, 40 μM Incubation Time: 30 hours Result: Attenuated p75NTR- and NRAGE-induced c-Jun phosphorylation and caspase-3 cleavage, and the levels of c-Jun protein. |
| 参考文献： |
1. Salehi AH, et, al. AEG3482 is an antiapoptotic compound that inhibits Jun kinase activity and cell death through induced expression of heat shock protein 70. Chem Biol. 2006 Feb;13(2):213-23. |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
-80℃（运输条件：冰袋低温运输） |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
3.567 ml |
17.837 ml |
35.674 ml |
| 5 mM |
0.713 ml |
3.567 ml |
7.135 ml |
| 10 mM |
0.357 ml |
1.784 ml |
3.567 ml |
| 50 mM |
0.071 ml |
0.357 ml |
0.713 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
