| 产品描述： | ITX3 is a specific and nontoxic inhibitor of TrioN (N-terminal GEF domain of the multidomain Trio protein) with an IC50 value of 76 μM. ITX3 can be used for the research of agent |
| 靶点： |
IC50: 76 μM (TrioN) |
| 体外研究： |
ITX3 (5, 10, 25, 50 and 100 μM; 24 h) inhibits TrioN signaling. ITX3 (50 μM; 1 h) specifically inhibits TrioN. ITX3 (100 μM; 36 h) inhibits nerve growth factor-induced neurite outgrowth in PC12 cells. ITX3 (1, 10 and 100 μM) represses Rac1 activity and dose-dependently up-regulates the E-cadherin protein level and phospho-p38 signal in Tara-KD cells. Western Blot Analysis Cell Line: TrioN-, GEF337-, Tiam1- and Vav2-expressing HEK293 cell lines Concentration: 50 μM Incubation Time: 1 hour Result: Decreased TrioN-induced Rac activity. |
| 参考文献： |
1. Bouquier N, et al. A cell active chemical GEF inhibitor selectively targets the Trio/RhoG/Rac1 signaling pathway. Chem Biol. 2009 Jun 26;16(6):657-66. 2. Yano T, et al. Tara up-regulates E-cadherin transcription by binding to the Trio RhoGEF and inhibiting Rac signaling. J Cell Biol. 2011 Apr 18;193(2):319-32. |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
-80℃（运输条件：冰袋低温运输） |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.692 ml |
13.461 ml |
26.921 ml |
| 5 mM |
0.538 ml |
2.692 ml |
5.384 ml |
| 10 mM |
0.269 ml |
1.346 ml |
2.692 ml |
| 50 mM |
0.054 ml |
0.269 ml |
0.538 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京