| 产品描述： | SM-7368 is a potent NF-kB inhibitor that targets downstream of MAPK p38 activation. SM-7368 inhibits TNF-α-induced MMP-9 upregulation. SM-7368 can be used for the research of chemotherapies targeting TNF-α-mediated tumor invasion and metastasis |
| 靶点： |
NF-κB;MMP-9;p38 MAP kinase |
| 体外研究： |
SM-7368 (5 μM) targets downstream of MAPK p38 activation in the human colon derived crypt like HT-29 and Caco-2 epithelial cell lines. SM-7368 inhibits TNF-α-induced MMP-9 upregulation in a concentration-dependent manner and shows maximal activity at 10 μM. SM-7368 inhibits TNF-α-induced MMP-9 mRNA transcript accumulation and protein expression. SM-7368 strongly inhibits TNF-α-induced NF-κB activity but not AP-1 activity. SM-7368 strongly inhibits the TNF-α-induced invasion of HT1080 human fibrosarcoma cell line. SM-7368 (10-25 μM) greatly inhibits TNF-α (20 ng/mL)-induced MMP-9 upregulation. 10 μM of SM-7368 almost completely abrogates this upregulation. Western Blot Analysis Cell Line: HT1080 human fibrosarcoma cells Concentration: 0, 1, 5, 10, 15, 20, and 25 μM Incubation Time: 24 hours Result: Greatly inhibited TNF-α (20 ng/mL)-induced MMP-9 upregulation in a concentration-dependent manner. |
| 参考文献： |
1. Sabine Kuntz, et al. Carbonyl compounds methylglyoxal and glyoxal affect interleukin-8 secretion in intestinal cells by superoxide anion generation and activation of MAPK p38. Mol Nutr Food Res. 2010 Oct;54(10):1458-67. 2. Ha Young Lee, et al. A small compound that inhibits tumor necrosis factor-alpha-induced matrix metalloproteinase-9 upregulation. Biochem Biophys Res Commun. 2005 Oct 21;336(2):716-22. |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
-80℃（运输条件：冰袋低温运输） |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
3.042 ml |
15.212 ml |
30.424 ml |
| 5 mM |
0.608 ml |
3.042 ml |
6.085 ml |
| 10 mM |
0.304 ml |
1.521 ml |
3.042 ml |
| 50 mM |
0.061 ml |
0.304 ml |
0.608 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
