| 产品描述： | WAY-100635 is a specific and effective 5-HT receptor antagonist (IC50=0.95 nM). |
| 靶点： |
5-HT Receptor |
| 体内研究： |
WAY 100635所产生的拮抗作用通过增加5-HT的浓度至300 μM,IC50为0.95 nM |
| 细胞实验： |
Extracellular recordings are made with glass microelectrodes filled with 2 M NaC1 (12 MΩ-15 MΩ). Cells are identified as 5-HT neurons according to the following criteria: biphasic action potentials of 2 msec to 3 msec in duration, slow (0.5 Hz - 2.0 Hz) and regular pattern of discharge. Firing is evoked in the otherwise silent neurons by adding the alpha-l adrenergic agonist phenylephrine (3 μM) to the superfusing ACSF. Baseline activity is recorded for at least 10 minutes before application of the different drugs. The electric signals are fed into a high-input impedance amplifier, an oscilloscope and an electronic ratemeter triggered by individual action potentials connected to an A/D converter and a personal computer. Using dedicated software, the integrated firing rate is recorded, computed and displayed on a chart recorder as consecutive 10-sec samples. The effects of agonists are evaluated by comparing the mean discharge frequency recorded during the 2 minutes that preceded WAY 100635 application with th |
| 参考文献： |
1.Corradetti R, et al. J Pharmacol Exp Ther. 1996, 278(2), 679-688. |
| 溶解性： |
Soluble in DMSO、Ethanol |
| 保存条件： |
-80℃（运输条件：冰袋低温运输） |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
1.857 ml |
9.283 ml |
18.565 ml |
| 5 mM |
0.371 ml |
1.857 ml |
3.713 ml |
| 10 mM |
0.186 ml |
0.928 ml |
1.857 ml |
| 50 mM |
0.037 ml |
0.186 ml |
0.371 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京