| 产品描述： | TC-H 106 (Pimelic Diphenylamide 106)是一种慢速、紧密结合的I类histone deacetylases(HDAC)抑制剂，对HDAC1, HDAC2, HDAC3的Ki值分别为148 nM, 约102 nM, 14 nM |
| 靶点： |
HDAC3(Cell-free assay):14 nM(Ki);HDAC2(Cell-free assay):~102 nM(Ki)HDAC1(Cell-free assay):148 nM(Ki) |
| 体内研究： |
Pimelic Diphenylamide 106 (50 mg/kg，i.p.；每天 1 次持续 3 天) 降低认知障碍幼鼠的过度活跃。 |
| 细胞实验： |
Cell lines: lymphoblastoid cell line Concentrations: 2 μM Incubation Time: 24 h Method: Cells are cultured in RPMI 1640 media with 10% fetal bovine serum and 10 mM HEPES, at 37 °C in 5% CO2. After the 5th split, the cells are treated with inhibitor TC-H 106 (2 μM) for 24 h, in culture medium. 24 h after treatment, the cells are washed twice with Hanks' balanced salts buffer to remove the inhibitor. A portion of the cell population is then harvested immediately after washing, and referred to as a time 0 point (24 h treatment point), and the rest of the cells are then re-cultured in cell culture media without added inhibitor. The re-cultured cells and controls are then harvested every hour for a total of 7 h. |
| 参考文献： |
1. Chou CJ, et al. J Biol Chem. 2008 19;283(51):35402-9. |
| 溶解性： |
Soluble in DMSO、Ethanol |
| 保存条件： |
-80℃（运输条件：冰袋低温运输） |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.946 ml |
14.731 ml |
29.461 ml |
| 5 mM |
0.589 ml |
2.946 ml |
5.892 ml |
| 10 mM |
0.295 ml |
1.473 ml |
2.946 ml |
| 50 mM |
0.059 ml |
0.295 ml |
0.589 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京