| 产品描述： | NS3694, a diarylurea compound, is an apoptosome inhibitor. NS3694 inhibits apoptosome formation and caspase activation |
| 靶点： |
Apoptosis |
| 体外研究： |
When cell extracts are stimulated with cytochrome c and dATP in the presence of NS3694 (10-100 μM; 120 min), a dose-dependent inhibition of caspase 9, 3, and 7 processing, DFF45/ICAD and PARP cleavage, and DEVDase activity is observed. NS3694 (10-100 μM) inhibits TNF-induced effector caspase activation and apoptosis in MCF-casp3 cells. NS3694 specifically suppresses the cytochrome C-induced formation of apoptosome complex and caspase-9 activation, thus inhibiting the mitochondria-mediated apoptosis. NS3694 inhibits the formation of the apoptosome Apaf-1 by blocking the activation of the initiator caspase-9 Western Blot Analysis Cell Line: HeLa cells Concentration: 10 μM, 50 μM, 100 μM Incubation Time: 120 min Result: Inhibited cytochrome c- and dATP-induced DEVDase activation and processing of caspases and caspase substrates. |
| 参考文献： |
1. Ulrik Lademann, et al. Diarylurea compounds inhibit caspase activation by preventing the formation of the active 700-kilodalton apoptosome complex. Mol Cell Biol. 2003 Nov;23(21):7829-37. |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
-80℃（运输条件：冰袋低温运输） |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.788 ml |
13.939 ml |
27.878 ml |
| 5 mM |
0.558 ml |
2.788 ml |
5.576 ml |
| 10 mM |
0.279 ml |
1.394 ml |
2.788 ml |
| 50 mM |
0.056 ml |
0.279 ml |
0.558 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京