| 产品描述： | Endovion (NS3728) is a pharmacological anion channel inhibitor (like chloride channel) and the specific VRAC/VSOAC blocker. Endovion (NS3728) is also an Anoctamin-1 (ANO 1) channel inhibitor |
| 靶点： |
Chloride channel, VRAC/VSOAC.ANO1 |
| 体外研究： |
Endovion (NS3728, 10-100 μM) reduces TNFα-induced apoptosis and increases p53-protein level as well as downstream signaling, e.g., expression of p21Waf1/Cip1, Bax, Noxa, MDM2, and activation of Caspase-9/-3 in Cisplatin-sensitive cells. Endovion (NS3728, 10 μM) inhibits cell proliferation in Capan-1, AsPC-1 and BxPC-3 cell lines. Western Blot Analysis Cell Line: Wild-type, resistant, and transiently transfected A2780 cells. Concentration: 10-100 μM. Incubation Time: 18 or 4.5 h. Result: Reduced the maximal taurine rate constant more than 90% compared with the untreated control cells.Resulted in an increased LRRC8A protein expression.Significantly reduceed p53 and p21Waf1/Cip1 protein level in A2780WT cells. Cell Proliferation Assay Cell Line: Capan-1, AsPC-1, BxPC-3 and H6c7 cell lines. Concentration: 10 μM. Incubation Time: 24 h. Result: Resulted in the most pronounced inhibition in all cell lines with 77 ± 26 % in Capan-1, 67 ± 9 % in AsPC-1, and 54 ± 8 % in BxPC-3 cells at +67 mV. |
| 参考文献： |
1. Sørensen BH, et al. Downregulation of LRRC8A protects human ovarian and alveolar carcinoma cells against CDDP-induced expression of p53, MDM2, p21Waf1/Cip1, and Caspase-9/-3 activation. Am J Physiol Cell Physiol. 2016 Jun 1;310(11):C857-73. 2. Sauter DR, et al. ANO1 (TMEM16A) in pancreatic ductal adenocarcinoma (PDAC). Pflugers Arch. 2015 Jul;467(7):1495-1508. |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
-80℃（运输条件：冰袋低温运输） |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.019 ml |
10.097 ml |
20.195 ml |
| 5 mM |
0.404 ml |
2.019 ml |
4.039 ml |
| 10 mM |
0.202 ml |
1.01 ml |
2.019 ml |
| 50 mM |
0.04 ml |
0.202 ml |
0.404 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京