| 产品描述： | LCS3 is a reversible and uncompetitive glutathione disulfide reductase (GSR) and thioredoxin reductase 1 (TXNRD1) inhibitor (IC50=3.3 µM and 3.8 µM, respectively). LCS3 shows anti-tumor activity, and induces apoptosis. LCS3 can be used in lung adenocarcinoma (LUAD) research |
| 体外研究： |
LCS3 (5 nM-10 µM; 96 h) inhibits lung cancer cell lines, but not non-transformed lung cells. LCS3 (3 µM; 3, 6, and 12 h) induces ROS and NRF2 pathway activation in sensitive lung adenocarcinoma (LUAD) cells. LCS3 (3 µM; 96 h) selectively kills lung adenocarcinoma (LUAD) cell lines, in part through the induction of apoptosis. Cell Viability Assay Cell Line: Non-small cell lung cancer (NSCLC) cells and non-transformed lung cells Concentration: 5 nM-10 µM Incubation Time: 96 hours Result: Inhibited the growth of 24/25 NSCLC cell lines at low micromolar concentrations (IC50<5 µM), both of the non-transformed lung cell lines were relatively insensitive (IC50>10 µM). Cell Viability Assay Cell Line: H23 and H1650 cells Concentration: 3 µM Incubation Time: 3, 6, and 12 hours Result: Responded to LCS3 by accumulating ROS and activating the NRF2 transcription program. Apoptosis Analysis Cell Line: lung adenocarcinoma (LUAD) cells Concentration: 3 µM Incubation Time: 96 hours Result: Increased cleavage of caspase 3, caspase 7 and/or PARP1 in all LCS3-sensitive LUAD cell lines. Western Blot Analysis Cell Line: H23 and H1650 cells Concentration: 3 µM Incubation Time: 24 hours Result: Increased the protein levels of NRF2 and of the products of selected downstream targets of NRF2 in both cell lines. |
| 参考文献： |
1. Fraser D Johnson, et al. Characterization of a small molecule inhibitor of disulfide reductases that induces oxidative stress and lethality in lung cancer cells. Cell Rep. 2022 Feb 8;38(6):110343. |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
-80℃（运输条件：冰袋低温运输） |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
3.75 ml |
18.752 ml |
37.504 ml |
| 5 mM |
0.75 ml |
3.75 ml |
7.501 ml |
| 10 mM |
0.375 ml |
1.875 ml |
3.75 ml |
| 50 mM |
0.075 ml |
0.375 ml |
0.75 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京