| 产品描述： | MitoBloCK-10 (MB-10) is the first small molecule modulator to attenuate protein-associated motor (PAM) complex activity. MitoBloCK-10 (MB-10) inhibits Tim44 (C-terminal domain) binding to the precursor and to Hsp70 |
| 靶点： |
HSP |
| 体外研究： |
MB-10 (100 μM) 是蛋白质输入线粒体的潜在衰减剂。 MB-10 抑制使用 TIM23 输入通路的底物输入。 MB-10 (0-100 μM) 抑制蛋白质输入哺乳动物线粒体。 Cell Viability Assay Cell Line: A plasmid expressing Su9-Ura3-myc was integrated at the LEU2 locus in WT and tim23-2 strains. Concentration: 100 μM. Incubation Time: 30 min. Result: WT strain expressing Su9-Ura3 failed to grow, whereas the tim23-2 mutant expressing Su9-Ura3 grew much faster. The strains grew at a similar rate when the medium was supplemented with uracil. Cell Viability Assay Cell Line: HeLa cells. Concentration: 0-100 μM. Incubation Time: 24 h. Result: Inhibited HeLa cells vibility, with an IC50 of 17.2 μM. |
| 参考文献： |
1. Non Miyata, et al. Adaptation of a Genetic Screen Reveals an Inhibitor for Mitochondrial Protein Import Component Tim44. J Biol Chem. 2017 Mar 31;292(13):5429-5442. |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
-80℃（运输条件：冰袋低温运输） |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
3.41 ml |
17.049 ml |
34.098 ml |
| 5 mM |
0.682 ml |
3.41 ml |
6.82 ml |
| 10 mM |
0.341 ml |
1.705 ml |
3.41 ml |
| 50 mM |
0.068 ml |
0.341 ml |
0.682 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京