| 产品描述： | SBI-477 是一种 insulin signaling 的抑制剂，可使转录因子 MondoA 失活，从而导致胰岛素途径抑制剂 thioredoxin-interacting protein (TXNIP) 和 arrestin domain-containing 4 (ARRDC4) 的表达降低。SBI-477 抑制人骨骼肌细胞中 triacylglyceride (TAG) 的合成并增强基础葡萄糖的摄取 |
| 靶点： |
MondoA; TXNIP; ARRDC4; TAG |
| 体外研究： |
SBI-477, that coordinately inhibits triacylglyceride (TAG) synthesis and enhances basal glucose uptake in human skeletal myocytes, stimulates insulin signaling by deactivating the transcription factor MondoA, leading to reduced expression of the insulin pathway suppressors thioredoxin-interacting protein (TXNIP) and arrestin domain containing 4 (ARRDC4) |
| 体内研究： |
Cell lines: Primary human skeletal myotubes Concentrations: -- Incubation Time: 24 h Method: Primary human skeletal myotubes were grown and differentiated in 24-well plates. Cells were treated with the indicated concentration of SBI-477 for 24 h. Following compound treatment, cells were rinsed three times with PBS and then incubated in 125μM [3H]-palmitic acid (60 Ci/mmol) bound to fatty acid free albumin containing 1mM carnitine for 2 hours at 37℃. The cell medium was transferred to a tube containing cold 10% trichloroacetic acid (TCA). The tubes were centrifuged at 8,500 x g for 10 minutes at 4℃. The supernatant was immediately removed, mixed with 6N NaOH, and applied to ion-exchange resin. The eluate was collected, measured by liquid scintillation analyzer and normalized to total protein amount. The amount of cell protein was measured. |
| 参考文献： |
1. Byungyong Ahn, et al. MondoA coordinately regulates skeletal myocyte lipid homeostasis and insulin signaling. J Clin Invest. 2016 Sep 1;126(9):3567-79. |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
-80℃（运输条件：冰袋低温运输） |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.068 ml |
10.34 ml |
20.681 ml |
| 5 mM |
0.414 ml |
2.068 ml |
4.136 ml |
| 10 mM |
0.207 ml |
1.034 ml |
2.068 ml |
| 50 mM |
0.041 ml |
0.207 ml |
0.414 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京